LOCUS       sequence-85510-e        3136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Transferrin Receptor, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3136)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-TFR-20
FEATURES             Location/Qualifiers
     source          1..3136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2380..2389
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2386..3096
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2531..2549)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2799..2818
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggactcag atctcgagcg ccaccatgat ggatcaagct
       61 agatcagcat tctctaactt gtttggtgga gaaccattgt catatacccg gttcagcctg
      121 gctcggcaag tagatggcga taacagtcat gtggagatga aacttgctgt agatgaagaa
      181 gaaaatgctg acaataacac aaaggccaat gtcacaaaac caaaaaggtg tagtggaagt
      241 atctgctatg ggactattgc tgtgatcgtc tttttcttga ttggatttat gattggctac
      301 ttgggctatt gtaaaggggt agaaccaaaa actgagtgtg agagactggc aggaaccgag
      361 tctccagtga gggaggagcc aggagaggac ttccctgcag cacgtcgctt atattgggat
      421 gacctgaaga gaaagttgtc ggagaaactg gacagcacag acttcaccag caccatcaag
      481 ctgctgaatg aaaattcata tgtccctcgt gaggctggat ctcaaaaaga tgaaaatctt
      541 gcgttgtatg ttgaaaatca atttcgtgaa tttaaactca gcaaagtctg gcgtgatcaa
      601 cattttgtta agattcaggt caaagacagc gctcaaaact cggtgatcat agttgataag
      661 aacggtagac ttgtttacct ggtggagaat cctgggggtt atgtggcgta tagtaaggct
      721 gcaacagtta ctggtaaact ggtccatgct aattttggta ctaaaaaaga ttttgaggat
      781 ttatacactc ctgtgaatgg atctatagtg attgtcagag cagggaaaat cacctttgca
      841 gaaaaggttg caaatgctga aagcttaaat gcaattggtg tgttgatata catggaccag
      901 actaaatttc ccattgttaa cgcagaactt tcattctttg gacatgctca tctggggaca
      961 ggtgaccctt acacacctgg attcccttcc ttcaaccaca ctcagtttcc accatctcgg
     1021 tcatcaggat tgcctaatat acctgtccag acaatctcca gagctgctgc agaaaagctg
     1081 tttgggaata tggaaggaga ctgtccctct gactggaaaa cagactctac atgtaggatg
     1141 gtaacctcag aaagcaagaa tgtgaagctc actgtgagca atgtgctgaa agagataaaa
     1201 attcttaaca tctttggagt tattaaaggc tttgtagaac cagatcacta tgttgtagtt
     1261 ggggcccaga gagatgcatg gggccctgga gctgcaaaat ccggtgtagg cacagctctc
     1321 ctattgaaac ttgcccagat gttctcagat atggtcttaa aagatgggtt tcagcccagc
     1381 agaagcatta tctttgccag ttggagtgct ggagactttg gatcggttgg tgccactgaa
     1441 tggctagagg gatacctttc gtccctgcat ttaaaggctt tcacttatat taatctggat
     1501 aaagcggttc ttggtaccag caacttcaag gtttctgcca gcccactgtt gtatacgctt
     1561 attgagaaaa caatgcaaaa tgtgaagcat ccggttactg ggcaatttct atatcaggac
     1621 agcaactggg ccagcaaagt tgagaaactc actttagaca atgctgcttt ccctttcctt
     1681 gcatattctg gaatcccagc agtttctttc tgtttttgcg aggacacaga ttatccttat
     1741 ttgggtacca ccatggacac ctataaggaa ctgattgaga ggattcctga gttgaacaaa
     1801 gtggcacgag cagctgcaga ggtcgctggt cagttcgtga ttaaactaac ccatgatgtt
     1861 gaattgaacc tggactatga gaggtacaac agccaactgc tttcatttgt gagggatctg
     1921 aaccaataca gagcagacat aaaggaaatg ggcctgagtt tacagtggct gtattctgct
     1981 cgtggagact tcttccgtgc tacttccaga ctaacaacag atttcgggaa tgctgagaaa
     2041 acagacagat ttgtcatgaa gaaactcaat gatcgtgtca tgagagtgga gtatcacttc
     2101 ctctctccct acgtatctcc aaaagagtct cctttccgac atgtcttctg gggctccggc
     2161 tctcacacgc tgccagcttt actggagaac ttgaaactgc gtaaacaaaa taacggtgct
     2221 tttaatgaaa cgctgttcag aaaccagttg gctctagcta cttggactat tcagggagct
     2281 gcaaatgccc tctctggtga cgtttgggac attgacaatg agttttcaga attcggctcc
     2341 accggctcca ccggctccac cggcgcggat ccaccggtcg ccaccatggt gagcaagggc
     2401 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     2461 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     2521 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     2581 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     2641 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2701 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     2761 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     2821 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2881 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2941 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     3001 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     3061 cactccaccg gcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa
     3121 tcagccatac cacatt
//
