LOCUS       sequence-85515-e        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-UbiquitinC-C-18
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             47..754
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(192..210)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     460..479
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             809..1033
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 cgcatgccta gtgacgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg
       61 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
      121 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
      181 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
      241 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
      301 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
      361 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
      421 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
      481 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
      541 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
      601 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
      661 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
      721 ccactccacc ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc
      781 ttcgaattcg gcagcggtgg agagcggtca gatcttcgtg aagactctga ctggtaagac
      841 catcaccctc gaggttgagc ccagtgacac cattgagaat gtcaaggcaa agatccaaga
      901 taaggaaggc atccctcctg accagcagag gctgatcttt gctggaaaac agctggaaga
      961 tgggcgcacc ctgtctgact acaacatcca gaaagagtcc accctgcacc tggtactccg
     1021 tctcagaggt gggtgaggat ccaccggatc tagataactg atcataatca gccataccac
     1081 attgtagaag ttgcc
//
