LOCUS       sequence-85516-e        1207 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane , Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1207)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-UbiquitinC-N-18
FEATURES             Location/Qualifiers
     source          1..1207
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             137..364
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     regulatory      413..422
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             419..1129
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(564..582)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     832..851
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tggagttgtt gcaccaatca tcggacttca aagtcgtaac aactccgccc ccatgacgca
       61 aatggcggta gcgtgtacgg tgggaggtct tatattaagc agagctgttt agtgatcgtc
      121 agatccgcta gcgcacatgc agatcttcgt gaagactctg actggtaaga ccatcaccct
      181 cgaggttgag cccagtgaca ccattgagaa tgtcaaggca aagatccaag ataaggaagg
      241 catccctcct gaccagcaga ggctgatctt tgctggaaaa cagctggaag atgggcgcac
      301 cctgtctgac tacaacatcc agaaagagtc caccctgcac ctggtactcc gtctcagagg
      361 tgggggtgga attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat
      421 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
      481 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
      541 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
      601 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
      661 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
      721 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
      781 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
      841 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
      901 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
      961 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     1021 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     1081 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa gcggccgcga
     1141 ctctagatca taatcagcca taccacattt gtagaggttt tactgcttta aaaacctccc
     1201 caacacc
//
