LOCUS       sequence-85517-e        2088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2088)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-VASP-5
FEATURES             Location/Qualifiers
     source          1..2088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        43..94
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     45..65
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      123..132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             129..836
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(274..292)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     542..561
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
       61 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      121 gcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
      181 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
      241 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
      301 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
      361 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
      421 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
      481 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
      541 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
      601 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
      661 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
      721 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
      781 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtccg
      841 gactcagatc catgagcgag acggtcatct gttccagccg ggctactgtg atgctttatg
      901 atgacagcaa caagcgatgg ctccctgctg gcactggtcc ccaggccttc agccgcgtcc
      961 agatctacca caaccccact gctaactcct tccgagttgt tggccgcaag atgcagccgg
     1021 atcagcaggt ggttatcaac tgtgccatca ttcggggtgt caagtacaat caggccactc
     1081 ccatcttcca tcagtggcga gatgcccgcc aggtctgggg cctcaacttc ggcagcaagg
     1141 aggacgccat acagtttgca acaggcatgg ccaacgccct agaggccttg gaaggaggtg
     1201 ggcctccccc agccccagca ccccctgcct ggtctgccca gaatggtccc tccccagagg
     1261 agctggaaca acagaaaagg cagccggagc atatggagcg ccgggtctcc aatgcaggag
     1321 gcccacctgc tcccccagct gggggccctc ctccacctcc aggacctccc cctcctccag
     1381 gtcccccccc acccccaggt ctgccctcct caggggtatc tggggcaggt catggagcag
     1441 gggcagcccc accccctgca cccccactcc ctacaacaca gggccccaat agtgggggtt
     1501 ccggggcccc aggcctggct gctgccattg ctggagccaa actcaggaaa gtgagcaagc
     1561 aggaggaggc ctctgggggg cccctggccc ccaaagctga gaacagtcga agcactggtg
     1621 gggggcttat ggaagagatg aacgccatgc tggcccggag aagaaaagcc acacaggttg
     1681 gggagaagcc ccccaaagac gagtcagcca gtgaggagtc agaggcccga ctccctgccc
     1741 agagtgaacc tgtgagaaga ccctgggaga agaacagcac aaccttgcca aggatgaagt
     1801 cgtcctcttc tgtgactacc tccgaggccc acccctcgac gccctgctcc agtgatgact
     1861 ccgacttgga gagggtgaag caggagcttc tggaagaggt gcggaaggag ctacagaaaa
     1921 tgaaagagga aatcatcgaa gtctttgtcc aggagctgag gaagcggggt tctccttgag
     1981 cggccgccac cgcggtggag ctcggtaccc cgggtcgacc tgcagccaag cttcgaattc
     2041 tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agataact
//
