LOCUS       sequence-85518-e        1913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1913)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-VASP-M-5
FEATURES             Location/Qualifiers
     source          1..1913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             24..731
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(169..187)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     437..456
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgcc accatggtga gcaagggcga ggaggataac atggccatca
       61 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
      121 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
      181 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
      241 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
      301 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
      361 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
      421 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
      481 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
      541 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
      601 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
      661 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
      721 agctgtacaa gtccggactc agatctatga gcgagacggt catctgttcc agccgggcta
      781 ctgtgatgct ttatgatgac agcaacaagc gatggctccc tgctggcact ggtccccagg
      841 ccttcagccg cgtccagata taccacaacc ccactgctaa ctccttccga gttgttggcc
      901 gcaagatgca gccggatcag caggtggtta tcaactgtgc catcattcgg ggtgtcaagt
      961 acaatcaggc cactcccatc ttccatcagt ggcgagatgc ccgccaggtc tggggcctca
     1021 acttcggcag caaggaggac gccatacagt ttgcaacagg catggccaac gccctagagg
     1081 ccttggaagg aggtgggcct cccccagccc cagcaccccc tgcctggtct gcccagaatg
     1141 gtccctcccc agaggagctg gaacaacaga aaaggcagcc ggagcatatg gagcgccggg
     1201 tctccaatgc aggaggccca cctgctcccc cagctggggg ccctcctcca cctccaggac
     1261 ctccccctcc tccaggtccc cccccacccc caggtctgcc ctcctcaggg gtatctgggg
     1321 caggtcatgg agcaggggca gccccacccc ctgcaccccc actccctaca acacagggcc
     1381 ccaatagtgg gggttccggg gccccaggcc tggctgctgc cattgctgga gccaaactca
     1441 ggaaagtgag caagcaggag gaggcctctg gggggcccct ggcccccaaa gctgagaaca
     1501 gtcgaagcac tggtgggggg cttatggaag agatgaacgc catgctggcc cggagaagaa
     1561 aagccacaca ggttggggag aagcccccca aagacgagtc agccagtgag gagtcagagg
     1621 cccgactccc tgcccagagt gaacctgtga gaagaccctg ggagaagaac agcacaacct
     1681 tgccaaggat gaagtcgtcc tcttctgtga ctacctccga ggcccacccc tcgacgccct
     1741 gctccagtga tgactccgac ttggagaggg tgaagcagga gcttctggaa gaggtgcgga
     1801 aggagctaca gaaaatgaaa gaggaaatca tcgaagtctt tgtccaggag ctgaggaagc
     1861 ggggttctcc ttgagcggcc gcgactctag atcataatca gccataccac atg
//
