LOCUS       sequence-85519-e        1930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1930)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-VASP-N-10
FEATURES             Location/Qualifiers
     source          1..1930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1174..1183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1180..1890
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1325..1343)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1593..1612
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtcagatccg ctagcgatgc caccatgagc gagacggtca tctgttccag ccgggctact
       61 gtgatgcttt atgatgacag caacaagcga tggctccctg ctggcactgg tccccaggcc
      121 ttcagccgcg tccagatata ccacaacccc actgctaact ccttccgagt tgttggccgc
      181 aagatgcagc cggatcagca ggtggttatc aactgtgcca tcattcgggg tgtcaagtac
      241 aatcaggcca ctcccatctt ccatcagtgg cgagatgccc gccaggtctg gggcctcaac
      301 ttcggcagca aggaggacgc catacagttt gcaacaggca tggccaacgc cctagaggcc
      361 ttggaaggag gtgggcctcc cccagcccca gcaccccctg cctggtctgc ccagaatggt
      421 ccctccccag aggagctgga acaacagaaa aggcagccgg agcatatgga gcgccgggtc
      481 tccaatgcag gaggcccacc tgctccccca gctgggggcc ctcctccacc tccaggacct
      541 ccccctcctc caggtccccc cccaccccca ggtctgccct cctcaggggt atctggggca
      601 ggtcatggag caggggcagc cccaccccct gcacccccac tccctacaac acagggcccc
      661 aatagtgggg gttccggggc cccaggcctg gctgctgcca ttgctggagc caaactcagg
      721 aaagtgagca agcaggagga ggcctctggg gggcccctgg cccccaaagc tgagaacagt
      781 cgaagcactg gtggggggct tatggaagag atgaacgcca tgctggcccg gagaagaaaa
      841 gccacacagg ttggggagaa gccccccaaa gacgagtcag ccagtgagga gtcagaggcc
      901 cgactccctg cccagagtga acctgtgaga agaccctggg agaagaacag cacaaccttg
      961 ccaaggatga agtcgtcctc ttctgtgact acctccgagg cccacccctc gacgccctgc
     1021 tccagtgatg actccgactt ggagagggtg aagcaggagc ttctggaaga ggtgcggaag
     1081 gagctacaga aaatgaaaga ggaaatcatc gaagtctttg tccaggagct gaggaagcgg
     1141 ggttctccta gctccggagg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag
     1201 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     1261 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     1321 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     1381 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     1441 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     1501 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     1561 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     1621 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     1681 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     1741 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     1801 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     1861 accggcggca tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc
     1921 ataccacatt
//
