LOCUS       sequence-85523-e        2231 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Shaner NC, Patterson GH, Davidson MW
  TITLE     Advances in fluorescent protein technology
  JOURNAL   J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60
  PUBMED    18057027
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2007-12-15"; volume: "120(Pt 24)"; pages: "4247-6"
COMMENT     Sequence Label: mCherry-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1471..1480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1477..2187
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1622..1640)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1890..1909
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattcccatg
       61 tccaccaggt ccgtgtcctc gtcctcctac cgcaggatgt tcggcggccc gggcaccgcg
      121 agccggccga gctccagccg gagctacgtg actacgtcca cccgcaccta cagcctgggc
      181 agcgcgctgc gccccagcac cagccgcagc ctctacgcct cgtccccggg cggcgtgtat
      241 gccacgcgct cctctgccgt gcgcctgcgg agcagcgtgc ccggggtgcg gctcctgcag
      301 gactcggtgg acttctcgct ggccgacgcc atcaacaccg agttcaagaa cacccgcacc
      361 aacgagaagg tggagctgca ggagctgaat gaccgcttcg ccaactacat cgacaaggtg
      421 cgcttcctgg agcagcagaa taagatcctg ctggccgagc tcgagcagct caagggccaa
      481 ggcaagtcgc gcctggggga cctctacgag gaggagatgc gggagctgcg ccggcaggtg
      541 gaccagctaa ccaacgacaa agcccgcgtc gaggtggagc gcgacaacct ggccgaggac
      601 atcatgcgcc tccgggagaa attgcaggag gagatgcttc agagagagga agccgaaaac
      661 accctgcaat ctttcagaca ggatgttgac aatgcgtctc tggcacgtct tgaccttgaa
      721 cgcaaagtgg aatctttgca agaagagatt gcctttttga agaaactcca cgaagaggaa
      781 atccaggagc tgcaggctca gattcaggaa cagcatgtcc aaatcgatgt ggatgtttcc
      841 aagcctgacc tcacggctgc cctgcgtgac gtacgtcagc aatatgaaag tgtggctgcc
      901 aagaacctgc aggaggcaga agaatggtac aaatccaagt ttgctgacct ctctgaggct
      961 gccaaccgga acaatgacgc cctgcgccag gcaaagcagg agtccactga gtaccggaga
     1021 caggtgcagt ccctcacctg tgaagtggat gcccttaaag gaaccaatga gtccctggaa
     1081 cgccagatgc gtgaaatgga agagaacttt gccgttgaag ctgctaacta ccaagacact
     1141 attggccgcc tgcaggatga gattcagaat atgaaggagg aaatggctcg tcaccttcgt
     1201 gaataccaag acctgctcaa tgttaagatg gcccttgaca ttgagattgc cacctacagg
     1261 aagctgctgg aaggcgagga gagcaggatt tctctgcctc ttccaaactt ttcctccctg
     1321 aacctgaggg aaactaatct ggattcactc cctctggttg atacccactc aaaaaggaca
     1381 cttctgatta agacggttga aactagagat ggacaggtta tcaacgaaac ttctcagcat
     1441 cacgatgacc ttgaagggga tccaccggtc gccaccatgg tgagcaaggg cgaggaggat
     1501 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     1561 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     1621 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     1681 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     1741 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     1801 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     1861 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     1921 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     1981 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     2041 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     2101 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     2161 ggcggcatgg acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata
     2221 ccacattgag a
//
