LOCUS       sequence-85524-e        2238 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mCherry-Vimentin-C-18
FEATURES             Location/Qualifiers
     source          1..2238
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(179..197)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      361 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
      541 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctggca gcggtggagg cagcgcatcc
      781 ggcggaagcg gaagctccac caggtccgtg tcctcgtcct cctaccgcag gatgttcggc
      841 ggcccgggca ccgcgagccg gccgagctcc agccggagct acgtgactac gtccacccgc
      901 acctacagcc tgggcagcgc gctgcgcccc agcaccagcc gcagcctcta cgcctcgtcc
      961 ccgggcggcg tgtatgccac gcgctcctct gccgtgcgcc tgcggagcag cgtgcccggg
     1021 gtgcggctcc tgcaggactc ggtggacttc tcgctggccg acgccatcaa caccgagttc
     1081 aagaacaccc gcaccaacga gaaggtggag ctgcaggagc tgaatgaccg cttcgccaac
     1141 tacatcgaca aggtgcgctt cctggagcag cagaataaga tcctgctggc cgagctcgag
     1201 cagctcaagg gccaaggcaa gtcgcgcctg ggggacctct acgaggagga gatgcgggag
     1261 ctgcgccggc aggtggacca gctaaccaac gacaaagccc gcgtcgaggt ggagcgcgac
     1321 aacctggccg aggacatcat gcgcctccgg gagaaattgc aggaggagat gcttcagaga
     1381 gaggaagccg aaaacaccct gcaatctttc agacaggatg ttgacaatgc gtctctggca
     1441 cgtcttgacc ttgaacgcaa agtggaatct ttgcaagaag agattgcctt tttgaagaaa
     1501 ctccacgaag aggaaatcca ggagctgcag gctcagattc aggaacagca tgtccaaatc
     1561 gatgtggatg tttccaagcc tgacctcacg gctgccctgc gtgacgtacg tcagcaatat
     1621 gaaagtgtgg ctgccaagaa cctgcaggag gcagaagaat ggtacaaatc caagtttgct
     1681 gacctctctg aggctgccaa ccggaacaat gacgccctgc gccaggcaaa gcaggagtcc
     1741 actgagtacc ggagacaggt gcagtccctc acctgtgaag tggatgccct taaaggaacc
     1801 aatgagtccc tggaacgcca gatgcgtgaa atggaagaga actttgccgt tgaagctgct
     1861 aactaccaag acactattgg ccgcctgcag gatgagattc agaatatgaa ggaggaaatg
     1921 gctcgtcacc ttcgtgaata ccaagacctg ctcaatgtta agatggccct tgacattgag
     1981 attgccacct acaggaagct gctggaaggc gaggagagca ggatttctct gcctcttcca
     2041 aacttttcct ccctgaacct gagggaaact aatctggatt cactccctct ggttgatacc
     2101 cactcaaaaa ggacacttct gattaagacg gttgaaacta gagatggaca ggttatcaac
     2161 gaaacttctc agcatcacga tgaccttgaa taaggatcca ccggatctag ataactgatc
     2221 ataatcagcc ataccaca
//
