LOCUS       sequence-85525-e        2223 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2223)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Vimentin-N-18
FEATURES             Location/Qualifiers
     source          1..2223
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1470..1479
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1476..2186
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1621..1639)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1889..1908
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgcc accatgtcca ccaggtccgt gtcctcgtcc tcctaccgca
       61 ggatgttcgg cggcccgggc accgcgagcc ggccgagctc cagccggagc tacgtgacta
      121 cgtccacccg cacctacagc ctgggcagcg cgctgcgccc cagcaccagc cgcagcctct
      181 acgcctcgtc cccgggcggc gtgtatgcca cgcgctcctc tgccgtgcgc ctgcggagca
      241 gcgtgcccgg ggtgcggctc ctgcaggact cggtggactt ctcgctggcc gacgccatca
      301 acaccgagtt caagaacacc cgcaccaacg agaaggtgga gctgcaggag ctgaatgacc
      361 gcttcgccaa ctacatcgac aaggtgcgct tcctggagca gcagaataag atcctgctgg
      421 ccgagctcga gcagctcaag ggccaaggca agtcgcgcct gggggacctc tacgaggagg
      481 agatgcggga gctgcgccgg caggtggacc agctaaccaa cgacaaagcc cgcgtcgagg
      541 tggagcgcga caacctggcc gaggacatca tgcgcctccg ggagaaattg caggaggaga
      601 tgcttcagag agaggaagcc gaaaacaccc tgcaatcttt cagacaggat gttgacaatg
      661 cgtctctggc acgtcttgac cttgaacgca aagtggaatc tttgcaagaa gagattgcct
      721 ttttgaagaa actccacgaa gaggaaatcc aggagctgca ggctcagatt caggaacagc
      781 atgtccaaat cgatgtggat gtttccaagc ctgacctcac ggctgccctg cgtgacgtac
      841 gtcagcaata tgaaagtgtg gctgccaaga acctgcagga ggcagaagaa tggtacaaat
      901 ccaagtttgc tgacctctct gaggctgcca accggaacaa tgacgccctg cgccaggcaa
      961 agcaggagtc cactgagtac cggagacagg tgcagtccct cacctgtgaa gtggatgccc
     1021 ttaaaggaac caatgagtcc ctggaacgcc agatgcgtga aatggaagag aactttgccg
     1081 ttgaagctgc taactaccaa gacactattg gccgcctgca ggatgagatt cagaatatga
     1141 aggaggaaat ggctcgtcac cttcgtgaat accaagacct gctcaatgtt aagatggccc
     1201 ttgacattga gattgccacc tacaggaagc tgctggaagg cgaggagagc aggatttctc
     1261 tgcctcttcc aaacttttcc tccctgaacc tgagggaaac taatctggat tcactccctc
     1321 tggttgatac ccactcaaaa aggacacttc tgattaagac ggttgaaact agagatggac
     1381 aggttatcaa cgaaacttct cagcatcacg atgaccttga aagcagcggt ggagcaagcg
     1441 cagccagtgg tagcgcggat ccaccggtcg ccaccatggt gagcaagggc gaggaggata
     1501 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     1561 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     1621 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     1681 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     1741 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     1801 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     1861 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     1921 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     1981 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     2041 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     2101 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     2161 gcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
     2221 cac
//
