LOCUS       sequence-85528-e        2282 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2282)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-WASH1-C-18
FEATURES             Location/Qualifiers
     source          1..2282
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..744
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(182..200)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     450..469
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     RBS             1875..1883
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tttacgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat
       61 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
      121 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      181 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      241 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
      301 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      361 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
      421 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
      481 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
      541 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
      601 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
      661 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
      721 ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgaattcg
      781 tccgcaggcg gcggaagcac accggtgaag acccagtgct ccttggcggg ccagctttat
      841 gcagtgcccc tcatccagcc cgacctgcga cgagaggagg caatccagca agtggcagat
      901 gccctgcagt acctacagaa catctctgga gacatcttca gcaggatctc ccgacgagta
      961 gagctgagtc ggcgccagct gcaggccatt agtgagaggg tctccttggc ccaggctaag
     1021 atagagaaga tcaagggcag taagaaggcc atcaaggtct tttctagtgc caagtaccca
     1081 gcaccagagc acctgcagga atacggctcc atctttactg gcgccttgga ccctggtctg
     1141 cagagaagac cccgttacag gatccagagc aagcaccgcc cactggatga gcgggctctg
     1201 caggagaagc tgaaatactt tcccgtgtgt gtgaacacca agtcggagcc tgaggatgaa
     1261 gctgaggagg gacttggggg tcttcccagc aacatcagct ccatcagctc cctgctgctc
     1321 ttcaacacca cagagaatct gtataagaag tatgttttcc tggaccctct ggctggtgca
     1381 gtaacaaaaa cgcacacgat gctgggcaca gaggaggaga aattgttcga tgcccctctg
     1441 tctatcagca agagagagca gctggagcgg caggctccag aaaactactt ctacgtgcca
     1501 gacctgggcc aggttcctga gatcgacgtg ccatcctacc tacccgattt acccggcgtc
     1561 gcagacgacc tgatgtacag tgcggatttg ggccctggca ttgccccgtc tgctcctggt
     1621 gctatcccag agttgcctgc cttccacaca gaggtggcag aacctctcca gccagagcta
     1681 gagaatgaag tactgttggc agcaccgccc ccgcctcctc ccccaccgcc gccacctccc
     1741 ccagctccca cagcattggt cagtacccca cagccaccga tgttccctga catggcaact
     1801 gctgctggcc aggttgccag ggaagaagac tctagcagca gcatggccca cacagcctct
     1861 gttcagggag cccctaagga ggtggttgac ccctccagtg gccgggccac tctgctcgag
     1921 tccatccgcc aagctggggg cattggcaag gccaagctac gcagcgtgaa ggagcgcaaa
     1981 ctggagaaga agaaacagaa ggagcaggag caagtgagag ccacgagcca aggtggggac
     2041 ttgatgtcag atctcttcaa caaacttgtc atgaggcgca aaggtatctc tggaaaggga
     2101 cctagcactg gaaccagtga gggacctgga ggagccttct ctcgaatgtc agactccatc
     2161 ccaccgctgc cacccccaca gcagccggca ggagacgagg atgaggagga ctgggagtcc
     2221 taaggtaccg cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc
     2281 ac
//
