LOCUS       sequence-85529-e        2251 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2251)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-WASH1-N-18
FEATURES             Location/Qualifiers
     source          1..2251
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1103..1111
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      1497..1506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1503..2213
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1648..1666)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1916..1935
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgcc accatgacac cggtgaagac ccagtgctcc ttggcgggcc
       61 agctttatgc agtgcccctc atccagcccg acctgcgacg agaggaggca atccagcaag
      121 tggcagatgc cctgcagtac ctacagaaca tctctggaga catcttcagc aggatctccc
      181 gacgagtaga gctgagtcgg cgccagctgc aggccattag tgagagggtc tccttggccc
      241 aggctaagat agagaagatc aagggcagta agaaggccat caaggtcttt tctagtgcca
      301 agtacccagc accagagcac ctgcaggaat acggctccat ctttactggc gccttggacc
      361 ctggtctgca gagaagaccc cgttacagga tccagagcaa gcaccgccca ctggatgagc
      421 gggctctgca ggagaagctg aaatactttc ccgtgtgtgt gaacaccaag tcggagcctg
      481 aggatgaagc tgaggaggga cttgggggtc ttcccagcaa catcagctcc atcagctccc
      541 tgctgctctt caacaccaca gagaatctgt ataagaagta tgttttcctg gaccctctgg
      601 ctggtgcagt aacaaaaacg cacacgatgc tgggcacaga ggaggagaaa ttgttcgatg
      661 cccctctgtc tatcagcaag agagagcagc tggagcggca ggctccagaa aactacttct
      721 acgtgccaga cctgggccag gttcctgaga tcgacgtgcc atcctaccta cccgatttac
      781 ccggcgtcgc agacgacctg atgtacagtg cggatttggg ccctggcatt gccccgtctg
      841 ctcctggtgc tatcccagag ttgcctgcct tccacacaga ggtggcagaa cctctccagc
      901 cagagctaga gaatgaagta ctgttggcag caccgccccc gcctcctccc ccaccgccgc
      961 cacctccccc agctcccaca gcattggtca gtaccccaca gccaccgatg ttccctgaca
     1021 tggcaactgc tgctggccag gttgccaggg aagaagactc tagcagcagc atggcccaca
     1081 cagcctctgt tcagggagcc cctaaggagg tggttgaccc ctccagtggc cgggccactc
     1141 tgctcgagtc catccgccaa gctgggggca ttggcaaggc caagctacgc agcgtgaagg
     1201 agcgcaaact ggagaagaag aaacagaagg agcaggagca agtgagagcc acgagccaag
     1261 gtggggactt gatgtcagat ctcttcaaca aacttgtcat gaggcgcaaa ggtatctctg
     1321 gaaagggacc tagcactgga accagtgagg gacctggagg agccttctct cgaatgtcag
     1381 actccatccc accgctgcca cccccacagc agccggcagg agacgaggat gaggaggact
     1441 gggagtccag cggaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca
     1501 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     1561 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     1621 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     1681 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     1741 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     1801 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     1861 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     1921 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     1981 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     2041 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     2101 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     2161 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag taaagcggcc
     2221 gcgactctag atcataatca gccataccac a
//
