LOCUS       sequence-85530-e        2389 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2389)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-WASP-like-N-18
FEATURES             Location/Qualifiers
     source          1..2389
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1255..1281
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HRHHHHHHH"
     CDS             1261..1284
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             1264..1290
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHLH"
     regulatory      1635..1644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1641..2351
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1786..1804)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2054..2073
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgacgtcag atccgctagc gccaccatga atagtggccc tggccctgta ggaggcaggc
       61 ctgggggccg agggggacca gccgttcagc agaacattcc ttccaacctc ctccaggacc
      121 atgaaaacca gagactcttt gagcttcttg gccgaaaatg ctggacactg gctaccacag
      181 ttgttcagct ctacctggca ctgccccctg gagctgagca ctggaccatg gaacactgcg
      241 gggctgtgtg cttcgtgaag gataaccctc agaagtccta cttcatccgc ctttatggcc
      301 tacaggctgg tcggctactc tgggaacagg agctgtactc tcagctggtt tatctcactc
      361 ccaccccgtt cttccacact tttgctggag atgactgtca agtaggactg aactttgcgg
      421 atgagagtga agcccaggcc ttccgggcct tggtgcagga gaagatacaa aaaaggaatc
      481 agaggcaaag cggagaaaga cgccagctac caccaccacc agcaccaatc aatgaggaga
      541 gaagaggagg gctcccacct gtgcccccac acccgggtgg agatcatggg ggcccatcag
      601 gtggtccact atctctagga cttgtgacgg tcgacattca gaaccctgac atcacaagtt
      661 cacgataccg tgggctccct gcacctggcc ctggcccaac tgataagaaa cgctcaggga
      721 aaaagaagat cagcaaagct gatatcggag caccgagtgg attcaaacat gtcagccacg
      781 tgggctggga tccccagaat ggatttgatg tgaacaacct agacccggat ctgcggagct
      841 tgttctccag ggcaggaatc agcgaggccc agctcactga cgcagagacc tccaagctca
      901 tctacgattt tattgaggac cagggaggtc tagaggctgt ccggcaggag atgaggcgcc
      961 aagagccact cccaccacct ccgccgccat gcagaggagg aggaggagga ggaggaggag
     1021 gaggaggagg aggaggagga ggaggaggcc agcctctgag acctcctgtt gtggggagta
     1081 ataagggtcg ctcaggtcca ctgccccctg tacctatggg gggtgcccca cctccaccaa
     1141 caccacgagg gcccccacca ccaggccgag ggggtcctcc tccaccaccc cctccagcca
     1201 ctggacgatc tggaccacca cctcctccac tccctggagc tgggggacca ccagcaccgc
     1261 caccaccacc accaccacca ccacctccac cctgccctgg gagtgggccc gcccctcccc
     1321 cgctccctcc tactccagtg tctgggggga gcccagcacc tggtgggggc cggggtgcac
     1381 ttttggacca aatccggcag ggaattcagc tgaacaagac ccctggagct ctagagaact
     1441 cagtacagca accacccgcg cagcagtcag aaggcctagt aggtgccctg atgcatgtca
     1501 tgcagaagag gagtagagtc atccattcct cagatgaagg ggaggatcag accggcgagg
     1561 atgaagagga tgatgaatgg gatgacagcg gcggaagtgg tagcggggta ccgcgggccc
     1621 gggatccacc ggtcgccacc atggtgagca agggcgagga ggataacatg gccatcatca
     1681 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
     1741 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     1801 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
     1861 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
     1921 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     1981 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     2041 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     2101 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
     2161 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     2221 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
     2281 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     2341 tgtacaagta aagcggccgc gactctagat cataatcagc cataccaca
//
