LOCUS       sequence-85532-e        2530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2530)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1774..1783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1780..2490
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1925..1943)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2193..2212
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcgcta ccggactcag atctcgagct caagcttcga attccatggc ggccccccgc
       61 ccgtctcccg cgatccccgt ttcggtctcg gctccggctt tttacgcccc gcagaagaag
      121 ttcggccctg tggtggcccc aaagcccaaa gtgaatccct tccggcccag ggacagcgag
      181 cctcccccgg cacccggggc ccagcgcgca cagatgggcc gggtgggcga tattcccccg
      241 ccgcccccgg aagactttcc cctgcctcca cctccccttg ctggggatgg cgacgatgca
      301 gagggtgctc tgggaggtgc cttcccgccg ccccctcccc cgatcgagga atcatttccc
      361 cctgcgcctc tggaggagga gatcttccct tccccgccgc ctcctccgga ggaggaggga
      421 gggcctgagg cccccatacc gcccccacca cagcccaggg agaaggtgag cagtattgat
      481 ttggagatcg actctctgtc ctcactgctg gatgacatga ccaagaatga tcctttcaaa
      541 gcccgggtgt catctggata tgtgccccca ccagtggcca ctccattcag ttccaagtcc
      601 agtaccaagc ctgcagccgg gggcacagca cccctgcctc cttggaagtc cccttccagc
      661 tcccagcctc tgccccaggt tccggctccg gctcagagcc agacacagtt ccatgttcag
      721 ccccagcccc agcccaagcc tcaggtccaa ctccatgtcc agtcccagac ccagcctgtg
      781 tctttggcta acacccagcc ccgagggccc ccagcctcat ctccggctcc agcccctaag
      841 ttttctccag tgactcctaa gtttactcct gtggcttcca agttcagtcc tggagcccca
      901 ggtggatctg ggtcacaacc aaatcaaaaa ttggggcacc ccgaagctct ttctgctggc
      961 acaggctccc ctcaacctcc cagcttcacc tatgcccagc agagggagaa gccccgagtg
     1021 caggagaagc agcaccccgt gcccccaccg gctcagaacc aaaaccaggt gcgctcccct
     1081 ggggccccag ggcccctgac tctgaaggag gtggaggagc tggagcagct gacccagcag
     1141 ctaatgcagg acatggagca tcctcagagg cagaatgtgg ctgtcaacga actctgcggc
     1201 cgatgccatc aacccctggc ccgggcgcag ccagccgtcc gcgctctagg gcagctgttc
     1261 cacatcgcct gcttcacctg ccaccagtgt gcgcagcagc tccagggcca gcagttctac
     1321 agtctggagg gggcgccgta ctgcgagggc tgttacactg acaccctgga gaagtgtaac
     1381 acctgcgggg agcccatcac tgaccgcatg ctgagggcca cgggcaaggc ctatcacccg
     1441 cactgcttca cctgtgtggt ctgcgcccgc cccctggagg gcacctcctt catcgtggac
     1501 caggccaacc ggccccactg tgtccccgac taccacaagc agtacgcccc gaggtgctcc
     1561 gtctgctctg agcccatcat gcctgagcct ggccgagatg agactgtgcg agtggtcgcc
     1621 ctggacaaga acttccacat gaagtgttac aagtgtgagg actgcgggaa gcccctgtcg
     1681 attgaggcag atgacaatgg ctgcttcccc ctggacggtc acgtgctctg tcggaagtgc
     1741 cacactgcta gagcccagac ggatccaccg gtcgccacca tggtgagcaa gggcgaggag
     1801 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     1861 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     1921 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     1981 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     2041 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     2101 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     2161 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     2221 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     2281 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     2341 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     2401 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     2461 accggcggca tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc
     2521 ataccacatt
//
