LOCUS       sequence-85537-e       10990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lacZ-reporter vector, integration at amyE, ampr, cmr.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 10990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: pBS1ClacZ
FEATURES             Location/Qualifiers
     source          1..10990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    123..144
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(159..164,165..182,183..189)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    197..213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             231..242
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             249..926
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      971..1042
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1058..1085
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1092..1112
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(4942..4960)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5028..5132
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5133..5201,5202..5993)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5351..5370)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6164..6752
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6653..6672
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6906..6923
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6938..7078
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7004..7023)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7164..7186
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7180..7371)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     gap             8312..8421
                     /estimated_length=110
     primer_bind     9857..9876
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gaattcgcgg ccgcttctag agcaatacgc aaaccgcctc tccccgcgcg ttggccgatt
       61 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      181 cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag aaagaggaga
      241 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
      301 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
      361 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
      421 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
      481 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
      541 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
      601 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgatggtccg gttatgcaga
      661 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
      721 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
      781 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
      841 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
      901 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag
      961 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1021 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1081 ctgcgtttat atactagtag cggccgctgc agggatccac agtacataaa aaaggagaca
     1141 tgacgatggt cgttttacaa cgtgactggg tcgaccggga aaaccctggc gttacccaac
     1201 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     1261 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc
     1321 cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg
     1381 tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtaa
     1441 cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact
     1501 cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg
     1561 atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg
     1621 acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc
     1681 tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag gatatgtggc
     1741 ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg
     1801 atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag
     1861 ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg
     1921 aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg
     1981 gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg
     2041 aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg
     2101 aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc
     2161 tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg
     2221 gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact
     2281 ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc
     2341 gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga
     2401 atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg
     2461 tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc
     2521 acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg
     2581 tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg
     2641 cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc
     2701 tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta
     2761 acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg
     2821 gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc
     2881 cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga
     2941 acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca aaacaccagc
     3001 agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt
     3061 tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg
     3121 caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg
     3181 aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga
     3241 acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg
     3301 aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg
     3361 aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct
     3421 ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt
     3481 tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta
     3541 acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc
     3601 agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc
     3661 atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa
     3721 tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc
     3781 tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag
     3841 aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag
     3901 acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat
     3961 tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc
     4021 aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc
     4081 tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat
     4141 cggcggaatt aattccagct gagcgccggt cgctaccatt accagttggt ctggtgtcaa
     4201 aaataataat aaccgggcag gccatgtctg cccgtatttc gcgtaaggaa atccattatg
     4261 tactattaat tctgcgtgac atcccatcga tcagaccagt ttttaatttg tgtgtttcca
     4321 tgtgtccagt ttggaatact cttaacctca ttggaaatcg cggcataatc actggtggta
     4381 tgattgatga ccgcgtcaac aatgaccttt atgccatatt cttcagcggc tgcacacatt
     4441 tctttaaatt cttgttcagt acctaagtaa cggttgccaa tttgatacga tgtcggctga
     4501 tacagccagt accagttcga catgctttta tctccttgat tcccttcctt tacttggtta
     4561 atcggagatg tctgaatggc tgtatatcct gcatcatgaa tatccttcat attgtgtttt
     4621 aacgtattga acgaccaatt ccatgcatga agaatggttc cgcttttgat cgacggtgct
     4681 gtaagctcat tcgatttgtt cgccgtttca gcactcgcag ccgccggtcc tgccagaacc
     4741 aaatgaaaca gcaataaaaa tccagcgaat aacggcagta aagaggtttt gaatcgtttt
     4801 gcaaacattc ttgacactcc ttatttgatt ttttgaagac ttacttcgga gtcaaaaatc
     4861 cctcttactt cattcttccg cttcctcctt tcaaaccgat gtgaagactg gagaattttg
     4921 ttaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     4981 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5041 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5101 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5161 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5221 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5281 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5341 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     5401 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5461 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5521 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5581 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5641 ccataccaaa cgacgagcgt gacaccacga tgccggcagc aatggcaaca acgttgcgca
     5701 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5761 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5821 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5881 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5941 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6001 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6061 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6121 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6181 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6241 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6301 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6361 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6421 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6481 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6541 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6601 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6661 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6721 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6781 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6841 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6901 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     6961 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     7021 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     7081 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     7141 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     7201 accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca
     7261 gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg
     7321 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc
     7381 cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct
     7441 cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca
     7501 actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt
     7561 cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg
     7621 gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc
     7681 gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt
     7741 tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg
     7801 ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga
     7861 gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt
     7921 ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa
     7981 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg
     8041 cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg
     8101 ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa
     8161 gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc
     8221 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
     8281 ataatgggga aggccatcca gcctcgcgtc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn ngctccggcg caaatgcaga caatatcagc atccttgcag
     8461 ggtatgtttc tctttgatgt ctttttgttt gtgaagtatt tcacatttat attgtgcaac
     8521 acttcacaaa cttttgcaag agaaaagttt tgtctgattt atgaacaaaa aagaaaccat
     8581 cattgatggt ttctttcggt aagtcccgtc tagccttgcc ctcaatgggg aagagaaccg
     8641 cttaagcccg agtcattata taaaccattt agcacgtaat caaagccagg ctgattctga
     8701 ccgggcactt gggcgctgcc attattaaaa atcacttttg cgttggttgt atccgtgtcc
     8761 gcaggcagcg tcagcgtgta aattccgtct gcatttttag tcattggttt tccaggccaa
     8821 gatccggtca attcaattac tcggctccca tcatgtttat agatataagc atttacctgg
     8881 ctccaatgat tcggattttg atagccgatg gttttggccg acgctggatc tcttttaaca
     8941 aaactgtatt tctcggtcct cgttacacca tcactgttcg ttccttttaa catgatggtg
     9001 tatgttttgc caaattggat ctccttttcc gattgtgaat tgatctccat ccttaaacgc
     9061 ctgtcgtctg gtccattatt gatttgataa acggcttttg ttgtattcgc atctgcacgc
     9121 aaggtaatcg tcagttgatc attgaaagaa tgtgttacac ctgttttgta attctcaagg
     9181 aaaacatgag gcgcttttgc aatatcatca ggataaagca cagctacaga cctggcattg
     9241 atcgtgcctg tcagtttacc atcgttcact tgaaatgaac ccgctccagc tttattgtca
     9301 tacctgccat caggcaattt tgttgccgta ttgatagaga cagaggatga acctgcattt
     9361 gccagcacaa cgccatgtga gccgcgctga ttcataaata tctggttgtt tccattcggg
     9421 ttcgagagtt cctcaggctg tccagccatc acattgtgaa atctattgac cgcagtgata
     9481 gcctgatctt caaataaagc actcccgcga tcgcctattt ggcttttccc ccagatctgg
     9541 ggaacctcac accatttccg cctccctcag gtctggaaaa gaaaagaggc gtactgcctg
     9601 aacgagaagc tatcaccgcc cagcctaaac ggatatcatc atcgctcatc catgtcgatc
     9661 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     9721 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     9781 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     9841 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     9901 cggtgatgcc ggccacgatg cgtccggcgt agaggatctg gagctgtaat ataaaaacct
     9961 tcttcaacta acggggcagg ttagtgacat tagaaaaccg actgtaaaaa gtacagtcgg
    10021 cattatctca tattataaaa gccagtcatt aggcctatct gacaattcct gaatagagtt
    10081 cataaacaat cctgcatgat aaccatcaca aacagaatga tgtacctgta aagatagcgg
    10141 taaatatatt gaattacctt tattaatgaa ttttcctgct gtaataatgg gtagaaggta
    10201 attactatta ttattgatat ttaagttaaa cccagtaaat gaagtccatg gaataataga
    10261 aagagaaaaa gcattttcag gtataggtgt tttgggaaac aatttccccg aaccattata
    10321 tttctctaca tcagaaaggt ataaatcata aaactctttg aagtcattct ttacaggagt
    10381 ccaaatacca gagaatgttt tagatacacc atcaaaaatt gtataaagtg gctctaactt
    10441 atcccaataa cctaactctc cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt
    10501 tatcaccctt gtcactaaga aaataaatgc agggtaaaat ttatatcctt cttgttttat
    10561 gtttcggtat aaaacactaa tatcaatttc tgtggttata ctaaaagtcg tttgttggtt
    10621 caaataatga ttaaatatct cttttctctt ccaattgtct aaatcaattt tattaaagtt
    10681 catttgatat gcctcctaaa tttttatcta aagtgaattt aggaggctta cttgtctgct
    10741 ttcttcatta gaatcaatcc ttttttaaaa gtcaatatta ctgtaacata aatatatatt
    10801 ttaaaaatat cccactttat ccaattttcg tttgttgaac taatgggtgc tttagttgaa
    10861 gaataaagac cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag
    10921 cgaaaaatcc ttttctttct tatcttgata ataagggtaa ctattgccga tgataagctg
    10981 tcaaacatga
//
