LOCUS       sequence-85542-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Wang D, Zhang Z, Chanda B, Jackson MB
  TITLE     Improved probes for hybrid voltage sensor imaging.
  JOURNAL   Biophys J. 2010 Oct 6;99(7):2355-65. doi: 10.1016/j.bpj.2010.07.037.
  PUBMED    20923671
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2010.07"; journalName: "Biophys J"; date: "2010-10-6-
            6"; volume: "99"; issue: "7"; pages: "2355-65"
COMMENT     Sequence Label: hVoS 1.5 thy-1
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(200..304)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     372..390
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(428..450)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     550..569
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atcattggaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
       61 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      121 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      181 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      241 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      301 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
      361 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
      421 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
      481 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
      541 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
      601 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
      661 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccaggat caattctagg
      721 acttaaggta ggaaactaag tggctgaagg tagagagaga ataaggacag tgaacgagtg
      781 ggtggtgggg agcttagaca tatgggcaga tcccaggtct tccaggcttg ctgacttgcc
      841 agagga
//
