LOCUS       sequence-85546-e        2133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  10xHis-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: His10-tag
FEATURES             Location/Qualifiers
     source          1..2133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             46..75
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     misc_feature    85..105
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      106..163
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(187..204)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(358..946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(438..457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1129..1223)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1244..1903)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1818..1837
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2065..2083
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2087..2130)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 aattccgcgg ccgcttctag ataaggagga actactatgg ccggccatca tcatcatcat
       61 catcatcatc atcataccgg ttaatactag tagcggccgc tgcagtccgg caaaaaaggg
      121 caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca
      181 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      361 ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
      781 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
      841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
      901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
      961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1021 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1081 aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat tctcaccaat
     1141 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     1201 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
     1261 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
     1321 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     1381 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     1441 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
     1501 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     1561 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     1621 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     1681 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     1741 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     1801 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     1861 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     1921 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     1981 agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt
     2041 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa
     2101 aaatccttag ctttcgctaa ggatgatttc tgg
//
