LOCUS       sequence-85549-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Yamada KM
  TITLE     Yamada Lab Plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: FN 127
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..52
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        34..52
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     RBS             659..681
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(712..731)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(713..731)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(815..834)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttcgggctt tgttagcagc cgaattgacg cgcaattaac cctcactaaa gggaacaaaa
       61 gctggaattc tacatctggg atggtttgtc aatttctgtt cggtaattaa tggaaattgg
      121 cttgctgctt gcggggctgt ctccacggcc agtgacagca tacacagtga tggtataatc
      181 aactccaggt ttaaggccgc tgatggtagc tgtagacttg ctcccaggca cagtgaactc
      241 ctggacaggg ctatttcctc ctgtttctcc gtaagtgatc ctgtaatatc tcactgtgac
      301 agcaggagca tcccagctga tcagtaggct ggtgggggtc gcagcaacaa cttccaggtc
      361 cctcggaaca tcagaaactg ttgattgttg gccaatcaat aagggacttt cctctctgcc
      421 attaagagca acgatgctga ccacatactc tgtgcctgga gtgaggttgg tgagggtgat
      481 ggaattgtct gaaggagaca cccgatcttc tcgaggtctc ccactgaagt gctcgggatg
      541 atggcggatc ctgtagccag tgatggtggc tcgaggagca atccagtgca cagtaaaaga
      601 gttggcagta atatcagaaa agtcaatgcc agttggggaa tcaagaccca tatgtatatc
      661 tccttcttaa agttaaacaa aattatttct agagggaaac cgttgtggtc tccctatagt
      721 gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct acgccggacg catcgtggcc
      781 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
      841 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
      901 ggccccgtgg ccgggggact gttgggcgcc atc
//
