LOCUS       sequence-85554-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no 
            signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. 
            Plasmid confers ampicillin resistance to DH5-alpha..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Tchekanda E, Sivanesan D, Michnick SW
  TITLE     An infrared reporter to detect spatiotemporal dynamics of 
            protein-protein interactions.
  JOURNAL   Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 
            2014 Apr 20.
  PUBMED    24747815
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2934"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "641-4"
COMMENT     Sequence Label: p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(19..37)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    85..588
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        618..722
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
       61 taatggtttc ttagggtcct tttcatcacg tgctataaaa ataattataa tttaaatttt
      121 ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt
      181 ttttgttttc cgaagatgta aaagactcta gggggatcgc caacaaatac taccttttat
      241 cttgctcttc ctgctctcag gtattaatgc cgaattgttt catcttgtct gtgtagaaga
      301 ccacacacga aaatcctgtg attttacatt ttacttatcg ttaatcgaat gtatatctat
      361 ttaatctgct tttcttgtct aataaatata tatgtaaagt acgctttttg ttgaaatttt
      421 ttaaaccttt gtttattttt ttttcttcat tccgtaactc ttctaccttc tttatttact
      481 ttctaaaatc caaatacaaa acataaaaat aaataaacac agagtaaatt cccaaattat
      541 tccatcatta aaagatacga ggcgcgtgta agttacaggc aagcgatccg tcaggtggca
      601 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      661 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      721 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt tttgcgcatt tgccttccgt
      781 tttgctccca gaacgctgtg aagtaagagc tgagatcagt
//
