LOCUS       sequence-85560-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral cDNA/shRNA gateway destination vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Radulovich N, Leung L, Tsao MS
  TITLE     Modified gateway system for double shRNA expression and Cre/lox 
            based gene expression.
  JOURNAL   BMC Biotechnol. 2011 Mar 22;11:24.
  PUBMED    21418658
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011-03-22"; volume: "11"; pages: "24"
COMMENT     Sequence Label: pLKO.DEST.EGFP
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(114..238)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    318..435
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(559..582)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
       61 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
      121 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
      181 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg
      241 atgggcggcc gctctagagg gcccaagctt acgcgtgaat tctcgacctc gagacaaatg
      301 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
      361 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
      421 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc
      481 gagggggttg gggttgcgcc ttttccaagg ctagaattct accgggtagg ggaggcgctt
      541 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
      601 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
      661 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
      721 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
      781 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
      841 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
      901 ctcagg
//
