LOCUS       sequence-85561-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral cDNA/shRNA gateway destination vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Radulovich N, Leung L, Tsao MS
  TITLE     Modified gateway system for double shRNA expression and Cre/lox 
            based gene expression.
  JOURNAL   BMC Biotechnol. 2011 Mar 22;11:24.
  PUBMED    21418658
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011-03-22"; volume: "11"; pages: "24"
COMMENT     Sequence Label: pLKO.DEST.EGFP
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(99..105,106..123,124..129))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(154..278)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(315..563)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(373..392)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(543..563)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(861..905)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
       61 tcctgaaaat ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata
      121 aagtgtaaag cctggggtgc ctaatgcggc cgccatagtg actggatatg ttgtgtttta
      181 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
      241 ttttacgttt ctcgttcagc ttttttgtac aaacttgttg atgggctcga ggatcgcgtg
      301 catacctgcg gaccggtgtt tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa
      361 tactttcaag ttacggtaag catatgatag tccattttaa aacataattt taaaactgca
      421 aactacccaa gaaattatta ctttctacgt cacgtatttt gtactaatat ctttgtgttt
      481 acagtcaaat taattccaat tatctctcta acagccttgt atcgtatatg caaatatgaa
      541 ggaatcatgg gaaataggcc ctcggtgaag ggggcggccg ctcgaggcta gtctcgtgat
      601 cgataccgtc gagatccgtt cactaatcga atggatctgt ctctgtctct ctctccacct
      661 tcttcttcta ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac
      721 gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt
      781 cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt
      841 gttatgttaa accaattcca caaacttgcc catttatcta attccaataa ttcttgttca
      901 ttcttttctt gctggttttg cgattcttca attaaggagt gta
//
