LOCUS       sequence-85563-e        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral cDNA/shRNA gateway destination vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Radulovich N, Leung L, Tsao MS
  TITLE     Modified gateway system for double shRNA expression and Cre/lox 
            based gene expression.
  JOURNAL   BMC Biotechnol. 2011 Mar 22;11:24.
  PUBMED    21418658
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011-03-22"; volume: "11"; pages: "24"
COMMENT     Sequence Label: pLKO.DEST.hygro
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(140..469)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      154..289
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(208..227)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     454..474
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    820..937
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
       61 cagacgtcgc ggtgagttca ggctttttca tcacgtgctg atcagatccg aaaatggata
      121 tacaagctcc cgggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
      181 tctggaatag ctcagaggca gaggcggcct cggcctctgc ataaataaaa aaaattagtc
      241 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
      301 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
      361 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
      421 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagaa
      481 ttatcgaatt cctgcagccc gggggatcca ctagtgagac gtgcggcttc cgtttgtcac
      541 gtccggcacg ccgcgaaccg caaggaacct tcccgactta ggggcggagc aggaagcgtc
      601 gccggggggc ccacaagggt agcggcgaag atccgggtga cgctgcgaac ggacgtgaag
      661 aatgtgcgag acccagggtc ggcgccgctg cgtttcccgg aaccacgccc agagcagccg
      721 cgtccctgcg caaacccagg gctgccttgg aaaaggcgca accccaaccc cctcgagccg
      781 cggccaaagt ggatctctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat
      841 ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc
      901 tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata ctgccatttg
      961 tctcgaggtc gagaattcac gcgtaagctt gggccctcta gagcggc
//
