LOCUS       sequence-85564-e        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral cDNA/shRNA gateway destination vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Radulovich N, Leung L, Tsao MS
  TITLE     Modified gateway system for double shRNA expression and Cre/lox 
            based gene expression.
  JOURNAL   BMC Biotechnol. 2011 Mar 22;11:24.
  PUBMED    21418658
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011-03-22"; volume: "11"; pages: "24"
COMMENT     Sequence Label: pLKO.DEST.hygro
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(121..245)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    325..442
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(788..808)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
       61 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
      121 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
      181 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
      241 gtggttgatg ggcggccgct ctagagggcc caagcttacg cgtgaattct cgacctcgag
      301 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
      361 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      421 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc
      481 gcggctcgag ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac
      541 gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat
      601 tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg
      661 cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga
      721 cgtgacaaac ggaagccgca cgtctcacta gtggatcccc cgggctgcag gaattcgata
      781 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag
      841 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
      901 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
      961 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccatt
//
