LOCUS       sequence-85565-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral cDNA/shRNA gateway destination vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Radulovich N, Leung L, Tsao MS
  TITLE     Modified gateway system for double shRNA expression and Cre/lox 
            based gene expression.
  JOURNAL   BMC Biotechnol. 2011 Mar 22;11:24.
  PUBMED    21418658
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011-03-22"; volume: "11"; pages: "24"
COMMENT     Sequence Label: pLKO.DEST.YFP
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(114..238)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(299..318)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(300..318)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(329..346)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(329..345)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(338..360)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    412..529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
       61 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
      121 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
      181 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg
      241 atgggcggcc gctctagagg gcccaagctt acgcgtgcat gcgacgtcat agctctctcc
      301 ctatagtgag tcgtattata agctaggcac tggccgtcgt tttacaacgt cgtgactggg
      361 aaaactgcta gaattctcga cctcgagaca aatggcagta ttcatccaca attttaaaag
      421 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
      481 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
      541 cagggacagc agagatccac tttggccgcg gctcgagggg gttggggttg cgccttttcc
      601 aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca
      661 gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct
      721 tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag
      781 gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta
      841 ccctcgcaga cggacagcgc cagg
//
