LOCUS       sequence-85567-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral cDNA/shRNA gateway destination vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Radulovich N, Leung L, Tsao MS
  TITLE     Modified gateway system for double shRNA expression and Cre/lox 
            based gene expression.
  JOURNAL   BMC Biotechnol. 2011 Mar 22;11:24.
  PUBMED    21418658
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011-03-22"; volume: "11"; pages: "24"
COMMENT     Sequence Label: pLKO.DEST.YFP
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(94..100,101..118,119..124))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(149..273)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(300..548)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(358..377)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(528..548)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
       61 aaaatctcga cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg
      121 taaagcctgg ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta
      181 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
      241 cgtttctcgt tcagcttttt tgtacaaact tgttgatggg ctcgaggata tcaccggccg
      301 gtgtttcgtc ctttccacaa gatatataaa gccaagaaat cgaaatactt tcaagttacg
      361 gtaagcatat gatagtccat tttaaaacat aattttaaaa ctgcaaacta cccaagaaat
      421 tattactttc tacgtcacgt attttgtact aatatctttg tgtttacagt caaattaatt
      481 ccaattatct ctctaacagc cttgtatcgt atatgcaaat atgaaggaat catgggaaat
      541 aggccctcgg tgaagggggc ggccgctcga ggctagtctc gtgatcgata ccgtcgagat
      601 ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct
      661 tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat
      721 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag
      781 cctcctacta tcattatgaa taattttata taccacagcc aatttgttat gttaaaccca
      841 attccacaaa cttg
//
