LOCUS       sequence-85575-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO 
            knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 
            31939).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D,
            Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, 
            Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad
            S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, 
            Novershtern N, Hanna JH
  TITLE     Derivation of novel human ground state naive pluripotent stem cells.
  JOURNAL   Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 
            2013 Oct 30.
  PUBMED    24172903
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12745"; journalName: "Nature"; date: "2013-12-12- 
            12"; volume: "504"; issue: "7479"; pages: "282-6"
COMMENT     Sequence Label: FOR human OCT4 TALEN
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     75..94
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(233..820)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 tattgcaatg aaaataaatt tcctttatta gccagaagtc agatgctcaa ggggcttcat
       61 gatgtcccca taatttttgg cagagggaaa aagatctcag tggtatttgt gagccagggc
      121 attggccaca ccagccacca ccttctgata ggcagcctgc acctgaggag tgaattttaa
      181 gaaactttat tgccaaatgt ttgaacgatc ggggaaattc gagctcctat taaaagttta
      241 tctcaccgtt attaaatttc cttctcactt cctcaagtgt caatgtacca gctttaatca
      301 tttctccacc aatcaaaagc tcttctacac taagaacagc tccattacaa ttagtgatat
      361 gattcaatct tgtaagctga gctttgtagt ttcctttgaa atgaccactc acaaacaaga
      421 acttaaattc tgttactgaa gatggataga ctttccacca ttcattaggg ttgatatgct
      481 tgtttcttgt ttgattctct tcgacatatc tttgcatttc atctgcttga ccaattggaa
      541 gattataacc tcctgaatat gccttagtat caacgatcac accgtaatca ataggagatc
      601 cgacagtata aattgctccg tctggtttcc ttgatccacc caaatgttta ccacgataac
      661 cataaacctt cataaagaac tccatcacct tcatttcaag gattctatcc tgagttgaat
      721 ttcttgcgat ttcaatcaat tcaatatatt catgtggcac atatttcaat ttatgtctaa
      781 gttcagattt cttctcttcc aattcagatt tcactagctg ggatctagat atcggatccg
      841 ggaggccgcc ccagatcctg gtacgcgggc gttttaccct ccagctgagg ggcaaatgca
      901 gcgcatcgcg ctgttcggga ac
//
