LOCUS       sequence-85577-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BAC recombineering generated construct, used as a reporter for mouse
            Oct4 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D,
            Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, 
            Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad
            S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, 
            Novershtern N, Hanna JH
  TITLE     Derivation of novel human ground state naive pluripotent stem cells.
  JOURNAL   Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 
            2013 Oct 30.
  PUBMED    24172903
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12745"; journalName: "Nature"; date: "2013-12-12- 
            12"; volume: "504"; issue: "7479"; pages: "282-6"
COMMENT     Sequence Label: mouse deltaPE Oct4-GFP transgenic reporter
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    69..190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(80..99)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     134..153
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(362..383)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     711..730
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgctgaccca tcacccccac ctggtggggg tgagaaggcg aagtctgaag ccaggtgtcc
       61 agcacgctta agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
      121 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
      181 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
      241 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctaga
      301 gtcgcggccg gccgctttac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
      361 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg
      421 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
      481 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
      541 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
      601 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
      661 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
      721 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
      781 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgta
//
