LOCUS       sequence-85579-e         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of human LIN-41 / TRIM71 containing cysteine 
            to alanine point mutations at cysteine 12 and 15 and 3 N-terminal HA
            tags .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Worringer KA, Rand TA, Hayashi Y, Sami S, Takahashi K, Tanabe K, 
            Narita M, Srivastava D, Yamanaka S
  TITLE     The let-7/LIN-41 pathway regulates reprogramming to human induced 
            pluripotent stem cells by controlling expression of 
            prodifferentiation genes.
  JOURNAL   Cell Stem Cell. 2014 Jan 2;14(1):40-52. doi: 
            10.1016/j.stem.2013.11.001. Epub 2013 Nov 14.
  PUBMED    24239284
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.11.001"; journalName: "Cell Stem Cell"; date: 
            "2014-01-2- 2"; volume: "14"; issue: "1"; pages: "40-52"
COMMENT     Sequence Label: pMXs-hs-3xHA-C12AC15A-LIN-41
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      94..103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..195
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 ggctgccgac cccgggggtg gaccatcctc tagactgccg gatctagcta gttaattaag
       61 gatcccagtg tggtggtacg ggaattcctg caggccacca tggcataccc atacgatgtt
      121 cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta tccatatgac
      181 gttccagatt acgctatggc ttcgttcccc gagaccgatt tccagatcgc cttgctggcc
      241 aaggagatgt gcggctcgcc ggcgccgctc tcctccaact cgtccgcgtc gtcgtcctcc
      301 tcgcagacgt ccacgtcgtc ggggggcggc ggcgggggcc ctggggcggc ggcgcgccgc
      361 ctacacgtcc tgccctgcct gcacgccttc tgccgcccct gcctcgaggc gcaccggctg
      421 ccggcggcgg gcggcggcgc ggcgggagag ccgctcaagc tgcgctgccc cgtgtgcgac
      481 cagaaagtag tgctagccga ggcggcgggt atggacgcgc tgccttcgtc cgccttcctg
      541 cttagcaacc tgctcgacgc ggtggtggcc actgccgacg agccgccgcc caagaacggg
      601 cgcgccggcg ctccggcggg agcgggcggc cacagcaacc ac
//
