LOCUS       sequence-85609-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO 
            knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 
            31939) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D,
            Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, 
            Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad
            S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, 
            Novershtern N, Hanna JH
  TITLE     Derivation of novel human ground state naive pluripotent stem cells.
  JOURNAL   Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 
            2013 Oct 30.
  PUBMED    24172903
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12745"; journalName: "Nature"; date: "2013-12-12- 
            12"; volume: "504"; issue: "7479"; pages: "282-6"
COMMENT     Sequence Label: REV human OCT4 TALEN
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     76..95
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(234..821)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 ctattgcaat gaaaataaat ttcctttatt agccagaagt cagatgctca aggggcttca
       61 tgatgtcccc ataatttttg gcagagggaa aaagatctca gtggtatttg tgagccaggg
      121 cattggccac accagccacc accttctgat aggcagcctg cacctgagga gtgaatttta
      181 agaaacttta ttgccaaatg tttgaacgat cggggaaatt cgagctccta ttaaaagttt
      241 atctcaccgt tattaaattt ccttctcact tcctcaagtg tcaatgtacc agctttaatc
      301 atttctccac caatcaaaag ctcttctaca ctaagaacag ctccattaca attagtgata
      361 tgattcaatc ttgtaagctg agctttgtag tttcctttga aatgaccact cacaaacaag
      421 aacttaaatt ctgttactga agatggatag actttccacc attcattagg gttgatatgc
      481 ttgtttcttg tttgattctc ttcgacatat ctttgcattt catctgcttg accaattgga
      541 agattataac ctcctgaata tgccttagta tcaacgatca caccgtaatc aataggagat
      601 ccgacagtat aaattgctcc gtctggtttc cttgatccac ccaaatgttt accacgataa
      661 ccataaacct tcataaagaa ctccatcacc ttcatttcaa ggattctatc ctgagttgaa
      721 tttcttgcga tttcaatcaa ttcaatatat tcatgtggca catatttcaa tttatgtcta
      781 agttcagatt tcttctcttc caattcagat ttcactagct gggatctaga tatcggatcc
      841 gggaggccgc cccagatcct ggtacgcggg cgtt
//
