LOCUS       sequence-85623-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human TDG-K330A C-terminally fused to GyrA 
            intein, SUMO-E2 and GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSC-hTDG-K330A-IntE2
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataaccatgg aagcggagaa cgcgggcagc
       61 tattcccttc agcaagctca agctttttat acgtttccat ttcaacaact gatggctgaa
      121 gctcctaata tggcagttgt gaatgaacag caaatgccag aagaagttcc agccccagct
      181 cctgctcagg aaccagtgca agaggctcca aaaggaagaa aaagaaaacc cagaacaaca
      241 gaaccaaaac aaccagtgga acccaaaaaa cctgttgagt caaaaaaatc tggcaagtct
      301 gcaaaatcaa aagaaaaaca agaaaaaatt acagacacat ttaaagtaaa aagaaaagta
      361 gaccgtttta atggtgtttc agaagctgaa cttctgacca agactctccc cgatattttg
      421 accttcaatc tggacattgt cattattggc ataaacccgg gactaatggc tgcttacaaa
      481 gggcatcatt accctggacc tggaaaccat ttttggaagt gtttgtttat gtcagggctc
      541 agtgaggtcc agctgaacca tatggatgat cacactctac cagggaagta tggtattgga
      601 tttaccaaca tggtggaaag gaccacgccc ggcagcaaag atctctccag taaagaattt
      661 cgtgaaggag gacgtattct agtacagaaa ttacagaaat atcagccacg aatagcagtg
      721 tttaatggaa aatgtattta tgaaattttt agtaaagaag tttttggagt aaaggttaag
      781 aacttggaat ttgggcttca gccccataag attccagaca cagaaa
//
