LOCUS       sequence-85624-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human XRCC1-K176R C-terminally fused to GyrA
            intein, SUMO-E2 and GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSC-hXRCC1-K176R-IntE2
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataaccatgg gaccggagat ccgcctccgc
       61 catgtcgtgt cctgcagcag ccaggactcg actcactgtg cagaaaatct tctcaaggca
      121 gacacttacc gaaaatggcg ggcagccaag gcaggcgaga agaccatctc tgtggtccta
      181 cagttggaga aggaggagca gatacacagt gtggacattg ggaatgatgg ctcagctttc
      241 gtggaggtgc tggtgggcag ttcagctgga ggcgctgggg agcaagacta tgaggtcctt
      301 ctggtcacct catctttcat gtccccttcc gagagccgca gtggctcaaa ccccaaccgc
      361 gttcgcatgt ttgggcctga caagctggtc cgggcagccg ccgagaagcg ctgggaccgg
      421 gtcaaaattg tttgcagcca gccctacagc aaggactccc cctttggctt gagttttgta
      481 cggtttcata gccccccaga caaagatgag gcagaggccc cgtcccagaa ggtgacagtg
      541 accaagcttg gccagttccg tgtgagggag gaggatgaga gcgccaactc tctgaggccg
      601 ggggctctct tcttcagccg gatcaacaag acatccccag tcacagccag cgacccggca
      661 ggacctagct atgcagctgc taccctccag gcttctagtg ctgcctcctc agcctctcca
      721 gtctccaggg ccataggcag cacctccaag ccccaggagt ctcccaaagg gaagaggaag
      781 ttggatttga accaagaaga aaagaagacc cccagcaaac caccagccca gctgtcgcca
      841 tctgttcc
//
