LOCUS       sequence-85629-e         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal chromosomal tagging with stable GFP under control of GAL 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  TITLE     Pringle Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-TRP1-PGAL1-GFP (F64L, S65T, V163A)
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(62..772)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(102..122)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     717..745
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(814..837)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 tatttaataa taaaaatcat aaatcataag aaattcgctt atttagaagt ggcgcgccct
       61 atttgtatag ttcatccatg ccatgtgtaa tcccagcagc tgttacaaac tcaagaagga
      121 ccatgtggtc tctcttttcg ttgggatctt tcgaaagggc agattgtgtg gacaggtaat
      181 ggttgtctgg taaaaggaca gggccatcgc caattggagt attttgttga taatggtctg
      241 ctagttgaac gcttccatct tcaatgttgt gtctaatttt gaagttagct ttgattccat
      301 tcttttgttt gtctgccatg atgtatacat tgtgtgagtt atagttgtat tccaatttgt
      361 gcccaagaat gtttccatct tctttaaaat caataccttt taactcgatt ctattaacaa
      421 gggtatcacc ttcaaacttg acttcagcac gtgtcttgta gttcccgtca tctttgaaaa
      481 atatagttct ttcctgtaca taaccttcgg gcatggcact cttgaaaaag tcatgctgtt
      541 tcatgtgatc tgggtatctt gaaaagcatt gaacaccata agtgagagta gtgacaagtg
      601 ttggccatgg aacaggtagt tttccagtag tgcaaataaa tttaagggta agttttccgt
      661 atgttgcatc accttcaccc tctccactga cagaaaattt gtgcccatta acatcaccat
      721 ctaattcaac aagaattggg acaactccag tgaaaagttc ttctccttta ctgttaatta
      781 aagacatttt gagatccggg gttttttctc cttgacgtta aagtatagag gtatattaac
      841 aattttttgt tgatactttt attacatttg aataagaagt aatacaaacc gaaaatgttg
      901 aaagtattag ttaaagtggt tatgcagttt ttgcatttat atatctgtta atagatcaaa
      961 aatcatcgct tcgctgatta attacccca
//
