LOCUS       sequence-85630-e        1234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 355, Emission: 424.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1234)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Sirius-H2B-6
FEATURES             Location/Qualifiers
     source          1..1234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..456
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      469..478
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(520..541)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(781..800)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             940..1191
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKAHFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLESVTAAGITLGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgccaccat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc
      121 tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc
      181 aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc
      241 atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc
      301 gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag
      361 atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag
      421 ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtcgc caccatggtg
      481 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      541 gtaaacggcc acaggttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      601 ctgaccctga agctcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      661 accaccctgc aattcggcgt gctgtgcttc gcccgctacc ccgaccacat gaagcagcac
      721 gacttcttca agtccgccat gcccgatggc tacgtccagg agcgtaccat cttcttcaag
      781 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      841 cgcatcgagc tgaagggctt cgacttcaag gaggacggca acatcctggg gcacaagctg
      901 gagtacaacg ggataagctc aaacgtatat atcaccgccg acaagcagaa gaacggcatc
      961 aaggcccact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac
     1021 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1081 agcgtccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1141 gagtccgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaagcggcc
     1201 gcgactctag atcataatca gccataccac attg
//
