LOCUS       sequence-85631-e        1198 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 355, Emission: 424.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1198)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Sirius-H2B-C-10
FEATURES             Location/Qualifiers
     source          1..1198
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             500..751
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKAHFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLESVTAAGITLGMDELYK"
     CDS             782..1159
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaggttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgc aattcggcgt
      241 gctgtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgatggc tacgtccagg agcgtaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggctt
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacg ggataagctc
      481 aaacgtatat atcaccgccg acaagcagaa gaacggcatc aaggcccact tcaagatccg
      541 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcgtccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagtccgtga ccgccgccgg
      721 gatcactctc ggcatggacg agctgtacaa gtccggactc agatctggag gctccggagg
      781 cccagagcca gcgaagtctg ctcccgcccc gaaaaagggc tccaagaagg cggtgactaa
      841 ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc aaggagagct attccatcta
      901 tgtgtacaag gttctgaagc aggtccaccc tgacaccggc atttcgtcca aggccatggg
      961 catcatgaat tcgtttgtga acgacatttt cgagcgcatc gcaggtgagg cttcccgcct
     1021 ggcgcattac aacaagcgct cgaccatcac ctccagggag atccagacgg ccgtgcgcct
     1081 gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag ggtactaagg ccatcaccaa
     1141 gtacaccagc gctaagtaag gatccaccgg atctagataa ctgatcataa tcagccat
//
