LOCUS       sequence-85633-e        1480 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Clathrin Vesicles, Excitation: 384, Emission: 450.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1480)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAzurite-Clathrin-15
FEATURES             Location/Qualifiers
     source          1..1480
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNIYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFRTAAGITHGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga gccacggcgt
      241 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact tcaacagcca
      481 caacatctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttccgca ccgccgccgg
      721 gatcactcac ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc
      781 gaattctgca gtcgacatgg ctgatgactt tggcttcttc tcgtcgtcgg agagtggtgc
      841 cccggaggcg gcggaggagg acccggcggc cgccttcctg gcccagcagg agagcgagat
      901 tgcaggcata gagaacgacg agggcttcgg ggcacctgcc ggcagccatg cggcccccgc
      961 acagccgggc cccacgagtg gggctggttc tgaggacatg gggaccacag tcaatggaga
     1021 tgtgtttcag gaggccaacg gtcctgctga tggctacgca gccattgccc aggctgacag
     1081 gctgacccag gagcctgaga gcatccgcaa gtggcgagag gagcagagga aacggctgca
     1141 agagctggat gctgcatcta aggtcacgga acaggaatgg cgggagaagg ccaagaagga
     1201 cctggaggag tggaaccagc gccagagtga acaagtagag aagaacaaga tcaacaaccg
     1261 ggcatccgag gaggctttcg tgaaggaatc caaggaggag accccaggca cagagtggga
     1321 gaaggtggcc cagctatgtg acttcaaccc caagagcagc aagcagtgca aagatgtgtc
     1381 ccgcctgcgc tcggtgctca tgtccctgaa gcagacgcca ctgtcccgct agggatccac
     1441 cggatctaga taactgatca taatcagcca taccacattg
//
