LOCUS       sequence-85636-e        2170 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 384, Emission: 450.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2170)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAzurite-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2170
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1401..1410
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1407..1409,1410..1412,1413..2126)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNIYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFRTAAGITHGMDELYK"
     primer_bind     complement(1452..1473)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1713..1732)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcagatccgc tagcgctacc ggatcagtct cgagctcaag cttcgaattc ggcagcactt
       61 ttctttcatt ggggggaagg cgtgaggaaa gtaccaaaca gcagcagact tttaaacttt
      121 aaacagacag gtctgagagc ctgaactctc atttttcctt tgacttcagc ctccaaggag
      181 ttccaccaac tttggcgcgc cggcttcact ttcattaagt gaaagagagg tgcccagaca
      241 tgggtgactg gagtgccttg gggaagcttc tggacaaggt ccaagcctac tccaccgctg
      301 gagggaaggt gtggctgtca gtgctcttca tattcagaat cctgctcctg gggacagctg
      361 ttgagtcagc ttggggtgat gaacagtctg cctttcgctg taacactcaa caacctggct
      421 gcgaaaacgt ctgctatgac aagtccttcc ccatctctca cgtgcgcttc tgggtccttc
      481 agatcatatt cgtgtctgtg cccacactcc tgtacttggc ccatgtgttc tatgtgatga
      541 ggaaggaaga gaagctaaac aagaaagaag aggagctcaa agtggcccag actgacgggg
      601 tcaacgtgga gatgcacctg aagcagattg aaatcaagaa gttcaagtac gggattgaag
      661 agcacggcaa ggtgaaaatg aggggcggct tgctgagaac ctacatcatc agcatcctct
      721 tcaagtctgt cttcgaggtg gccttcctgc tcatccagtg gtacatctat gggttcagct
      781 tgagcgcggt ctacacctgc aagagagatc cctgccccca ccaggtagac tgcttcctct
      841 cacgtcccac ggagaaaacc atcttcatca tcttcatgct ggtggtgtcc ttggtgtctc
      901 tcgctttgaa catcattgag ctcttctacg tcttcttcaa aggcgttaag gatcgcgtga
      961 agggaagaag cgatccttac cacgccacca ctggcccact gagcccatca aaagactgcg
     1021 gatctccaaa atacgcctac ttcaatggct gctcctcacc aacggctcca ctctcgccta
     1081 tgtctcctcc tgggtacaag ctggttactg gtgacagaaa caattcctcg tgccgcaatt
     1141 acaacaagca agctagcgag caaaactggg cgaactacag cgcagagcaa aatcgcatgg
     1201 ggcaggccgg aagcaccatc tccaactcgc acgcccagcc gttcgatttc cccgacgaca
     1261 accagaatgc caaaaaagtt gctgctggac atgaactcca gccattagcc atcgtggacc
     1321 aacgaccttc cagcagagcc agcagccgcg ccagcagcag gcctcggcct gatgacctgg
     1381 agattgcgga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     1441 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1501 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     1561 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gagccacggc gtgcagtgct
     1621 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1681 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1741 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1801 aggaggacgg caacatcctg gggcacaagc tggagtacaa cttcaacagc cacaacatct
     1861 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     1921 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1981 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
     2041 ccaacgagaa gcgcgatcac atggtcctgc tggagttccg caccgccgcc gggatcactc
     2101 acggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc
     2161 acattgtaga
//
