LOCUS       sequence-85638-e        2175 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 384, Emission: 450.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2175)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAzurite-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..2175
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNIYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFRTAAGITHGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(341..360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      181 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga gccacggcgt
      241 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact tcaacagcca
      481 caacatctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
      541 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      601 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
      661 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttccgca ccgccgccgg
      721 gatcactcac ggcatggacg agctgtacaa gtccggactc agatctcgag tgcgtgagtg
      781 catctccatc cacgttggcc aggctggtgt ccagattggc aatgcctgct gggagctcta
      841 ctgcctggaa cacggcatcc agcccgatgg ccagatgcca agtgacaaga ccattggggg
      901 aggagatgac tccttcaaca ccttcttcag tgagacgggc gctggcaagc acgtgccccg
      961 ggctgtgttt gtagacttgg aacccacagt cattgatgaa gttcgcactg gcacctaccg
     1021 ccagctcttc caccctgagc agctcatcac aggcaaggaa gatgctgcca ataactatgc
     1081 ccgagggcac tacaccattg gcaaggagat cattgacctt gtgttggacc gaattcgcaa
     1141 gctggctgac cagtgcaccg gtcttcaggg cttcttggtt ttccacagct ttggtggggg
     1201 aactggttct gggttcacct ccctgctcat ggaacgtctc tcagttgatt atggcaagaa
     1261 gtccaagctg gagttctcca tttacccagc accccaggtt tccacagctg tagttgagcc
     1321 ctacaactcc atcctcacca cccacaccac cctggagcac tctgattgtg ccttcatggt
     1381 agacaatgag gccatctatg acatctgtcg tagaaacctc gatatcgagc gcccaaccta
     1441 cactaacctt aaccgcctta ttagccagat tgtgtcctcc atcactgctt ccctgagatt
     1501 tgatggagcc ctgaatgttg acctgacaga attccagacc aacctggtgc cctacccccg
     1561 catccacttc cctctggcca catatgcccc tgtcatctct gctgagaaag cctaccatga
     1621 acagctttct gtagcagaga tcaccaatgc ttgctttgag ccagccaacc agatggtgaa
     1681 atgtgaccct cgccatggta aatacatggc ttgctgcctg ttgtaccgtg gtgacgtggt
     1741 tcccaaagat gtcaatgctg ccattgccac catcaaaacc aagcgcagca tccagtttgt
     1801 ggattggtgc cccactggct tcaaggttgg catcaactac cagcctccca ctgtggtgcc
     1861 tggtggagac ctggccaagg tacagagagc tgtgtgcatg ctgagcaaca ccacagccat
     1921 tgctgaggcc tgggctcgcc tggaccacaa gtttgacctg atgtatgcca agcgtgcctt
     1981 tgttcactgg tacgtgggtg aggggatgga ggaaggcgag ttttcagagg cccgtgaaga
     2041 tatggctgcc cttgagaagg attatgagga ggttggtgtg gattctgttg aaggagaggg
     2101 tgaggaagaa ggagaggaat actaaggatc caccggatct agataactga tcataatcag
     2161 ccataccaca ttgag
//
