LOCUS       sequence-85640-e        3195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 383, Emission: 448.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3195)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBFP2-Beta-Catenin-20
FEATURES             Location/Qualifiers
     source          1..3195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(27..29,30..32,33..743)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(72..93)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      798..807
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             849..884
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
       61 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgaggg
      121 gcgagggcga gggcgatgcc accaacggca agctgaccct gaagttcatc tgcaccaccg
      181 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gagccacggc gtgcagtgct
      241 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      301 gctacgtcca ggagcgcacc atcttcttca aggacgacgg cacctacaag acccgcgccg
      361 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc gtcgacttca
      421 aggaggacgg caacatcctg gggcacaagc tggagtacaa cttcaacagc cacaacatct
      481 atatcatggc cgtcaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaacg
      541 tggaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      601 gccccgtgct gctgcccgac agccactacc tgagcaccca gtccgtgctg agcaaagacc
      661 ccaacgagaa gcgcgatcac atggtcctgc tggagttccg caccgccgcc gggatcactc
      721 tcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcgaattctg
      781 cagtcgacgg taccgcggcc accatggcta ctcaagctga cctgatggag ttggacatgg
      841 ccatggagcc ggacagaaaa gctgctgtca gccactggca gcagcagtct tacttggatt
      901 ctggaatcca ttctggtgcc accaccacag ctccttccct gagtggcaag ggcaaccctg
      961 aggaagaaga tgttgacacc tcccaagtcc tttatgaatg ggagcaaggc ttttcccagt
     1021 ccttcacgca agagcaagta gctgatattg acgggcagta tgcaatgact agggctcaga
     1081 gggtccgagc tgccatgttc cctgagacgc tagatgaggg catgcagatc ccatccacgc
     1141 agtttgacgc tgctcatccc actaatgtcc agcgcttggc tgaaccatca cagatgttga
     1201 aacatgcagt tgtcaatttg attaactatc aggatgacgc ggaacttgcc acacgtgcaa
     1261 ttcctgagct gacaaaactg ctaaacgatg aggaccaggt ggtagttaat aaagctgctg
     1321 ttatggtcca tcagctttcc aaaaaggaag cttccagaca tgccatcatg cgctcccctc
     1381 agatggtgtc tgccattgta cgcaccatgc agaatacaaa tgatgtagag acagctcgtt
     1441 gtactgctgg gactctgcac aacctttctc accaccgcga gggcttgctg gccatcttta
     1501 agtctggtgg catcccagcg ctggtgaaaa tgcttgggtc accagtggat tctgtactgt
     1561 tctacgccat cacgacactg cataatctcc tgctccatca ggaaggagct aaaatggcag
     1621 tgcgcctagc tggtggactg cagaaaatgg ttgctttgct caacaaaaca aacgtgaaat
     1681 tcttggctat tacaacagac tgccttcaga tcttagctta tggcaatcaa gagagcaagc
     1741 tcatcattct ggccagtggt ggaccccaag ccttagtaaa cataatgagg acctacactt
     1801 atgagaagct tctgtggacc acaagcagag tgctgaaagt gctgtctgtc tgctctagca
     1861 acaagccggc cattgtagaa gctggtggga tgcaggcact ggggcttcat ctgacagacc
     1921 caagtcagcg acttgttcaa aactgtcttt ggactctcag aaacctttca gatgcagcga
     1981 ctaagcagga agggatggaa ggcctccttg ggactctagt gcagcttctg ggttccgatg
     2041 atataaatgt ggtcacctgt gcagctggaa ttctctctaa cctcacttgc aataattaca
     2101 aaaacaagat gatggtgtgc caagtgggtg gcatagaggc tcttgtacgc accgtccttc
     2161 gtgctggtga cagggaagac atcactgagc ctgccatctg tgctcttcgt catctgacca
     2221 gccggcatca ggaagccgag atggcccaga atgccgttcg ccttcattat ggactgcctg
     2281 ttgtggttaa actcctgcac ccaccatccc actggcctct gataaaggca actgttggat
     2341 tgattcgaaa ccttgccctt tgcccagcaa atcatgcgcc tttgcgggaa cagggtgcta
     2401 ttccacgact agttcagctg cttgtacgag cacatcagga cacccaacgg cgcacctcca
     2461 tgggtggaac gcagcagcag tttgtggagg gcgtgcgcat ggaggagata gtagaagggt
     2521 gtactggagc tctccacatc cttgctcggg acgttcacaa ccggattgta atccgaggac
     2581 tcaataccat tccattgttt gtgcagttgc tttattctcc cattgaaaat atccaaagag
     2641 tagctgcagg ggtcctctgt gaacttgctc aggacaagga ggctgcagag gccattgaag
     2701 ctgagggagc cacagctccc ctgacagagt tactccactc caggaatgaa ggcgtggcaa
     2761 catacgcagc tgctgtccta ttccgaatgt ctgaggacaa gccacaggat tacaagaagc
     2821 ggctttcagt cgagctgacc agttccctct tcaggacaga gccaatggct tggaatgaga
     2881 ctgcagatct tggactggac attggtgccc agggagaagc ccttggatat cgccaggatg
     2941 atcccagcta ccgttctttt cactctggtg gatacggcca ggatgccttg gggatggacc
     3001 ctatgatgga gcatgagatg ggtggccacc accctggtgc tgactatcca gttgatgggc
     3061 tgcctgatct gggacacgcc caggacctca tggatgggct gcccccaggt gatagcaatc
     3121 agctggcctg gtttgatact gacctgtaaa agcttggatc caccggatct agataactga
     3181 tcataatcag ccata
//
