LOCUS       sequence-85643-e        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 383, Emission: 448.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBFP2-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        11..48
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      269..278
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(275..277,278..280,281..994)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(320..341)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcaaatgggc gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
       61 cgtcagatcc gctagcgcta ccggactcag atctggtacc cttgcagctt ccagttgtca
      121 ggaagagaag gctcccatgg atcctgtggt ggtgctgggg ctctgtctct cctgtttgct
      181 tctcctttca ctctggaaac agagctatgg gggagggaag cttcgaattc tgcagtcgac
      241 ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt
      301 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      361 cgtgaggggc gagggcgagg gcgatgccac caacggcaag ctgaccctga agttcatctg
      421 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga gccacggcgt
      481 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      541 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca cctacaagac
      601 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcgt
      661 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact tcaacagcca
      721 caacatctat atcatggccg tcaagcagaa gaacggcatc aaggtgaact tcaagatccg
      781 ccacaacgtg gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      841 cggcgacggc cccgtgctgc tgcccgacag ccactacctg agcacccagt ccgtgctgag
      901 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttccgca ccgccgccgg
      961 gatcactctc ggcatggacg agctgtacaa gtaagcggcc gcgactctag atcataatca
     1021 gccataccac atttgtagag gttttacttg ctttaaaaac ctcccacacc tccccctgaa
     1081 cctgaaacat aaaatgaatg caattgtgtt gtaacttgtt tatgcagctt ataatg
//
