LOCUS       sequence-85644-e        1681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 383, Emission: 
            448.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1681)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBFP2-EB3-7
FEATURES             Location/Qualifiers
     source          1..1681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      914..923
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(920..922,923..925,926..1639)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(965..986)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gctaccggac tcagatctcg agctcaagct tcgaattctg cagtcgactg ccaccatggc
       61 cgtcaatgtg tactccacat ctgtgaccag tgaaaatctg agtcgccatg atatgcttgc
      121 atgggtcaac gactccctgc acctcaacta taccaagata gaacagcttt gttcaggggc
      181 agcctactgc cagttcatgg acatgctctt ccccggctgt gtgcacttga ggaaagtgaa
      241 gttccaggcc aaactagagc atgaatacat ccacaacttc aaggtgctgc aagcagcttt
      301 caagaagatg ggtgttgaca aaatcattcc tgtagagaaa ttagtgaaag gaaaattcca
      361 agataatttt gagtttattc agtggtttaa gaaattcttt gacgcaaact atgatggaaa
      421 ggattacaac cctctgctgg cgcggcaggg ccaggacgta gcgccacctc ctaacccagg
      481 tgatcagatc ttcaacaaat ccaagaaact cattggcaca gcagttccac agaggacgtc
      541 ccccacaggc ccaaaaaaca tgcagacctc tggccggctg agcaatgtgg cccccccctg
      601 cattctccgg aagaatcctc catcagcccg aaatggcggc catgagactg atgcccaaat
      661 tcttgaactc aaccaacagc tggtggactt gaagctgaca gtggatgggc tggagaagga
      721 acgtgacttc tacttcagca aacttcgtga catcgagctc atctgccagg agcatgaaag
      781 tgaaaacagc cctgttatct caggcatcat tggcatcctc tatgccacag aggaaggatt
      841 cgcaccccct gaggacgatg agattgaaga gcatcaacaa gaagaccagg acgagtacct
      901 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      961 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtga ggggcgaggg
     1021 cgagggcgat gccaccaacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     1081 gcccgtgccc tggcccaccc tcgtgaccac cctgagccac ggcgtgcagt gcttcgcccg
     1141 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1201 ccaggagcgc accatcttct tcaaggacga cggcacctac aagacccgcg ccgaggtgaa
     1261 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcgtcgact tcaaggagga
     1321 cggcaacatc ctggggcaca agctggagta caacttcaac agccacaaca tctatatcat
     1381 ggccgtcaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acgtggagga
     1441 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1501 gctgctgccc gacagccact acctgagcac ccagtccgtg ctgagcaaag accccaacga
     1561 gaagcgcgat cacatggtcc tgctggagtt ccgcaccgcc gccgggatca ctctcggcat
     1621 ggacgagctg tacaagtaag cggccgcgac tctagatcat aatcagccat accacattga
     1681 g
//
