LOCUS       sequence-85645-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 383, Emission: 448.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBFP2-ER-5
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..63
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     14..34
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      158..167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(164..166,167..169,170..880)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(209..230)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
       61 gctggtttag tgaaccgtca gatccgctag cgccaccatg ctgctatccg tgccgttgct
      121 gctcggcctc ctcggcctgg ccgtcgccgc accggtcgcc accatggtga gcaagggcga
      181 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      241 caagttcagc gtgaggggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa
      301 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgag
      361 ccacggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
      421 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcac
      481 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      541 gaagggcgtc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt
      601 caacagccac aacatctata tcatggccgt caagcagaag aacggcatca aggtgaactt
      661 caagatccgc cacaacgtgg aggacggcag cgtgcagctc gccgaccact accagcagaa
      721 cacccccatc ggcgacggcc ccgtgctgct gcccgacagc cactacctga gcacccagtc
      781 cgtgctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttccgcac
      841 cgccgccggg atcactctcg gcatggacga gctgtacaag tccggaaagg acgagctgta
      901 agaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga taactgatca
      961 taatcagcca taccacattt gtagaggttt tact
//
