LOCUS       sequence-85650-e        1338 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Light Chain Myosin, Excitation: 383, Emission: 448.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1338)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBFP2-LC-Myosin-N-7
FEATURES             Location/Qualifiers
     source          1..1338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      574..583
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(580..582,583..585,586..1299)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(625..646)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttatgtcgag caagagagcc
       61 aaggccaaga ccaccaagaa gaggccccag agggctacgt ccaatgtctt cgccatgttt
      121 gaccagtccc agatccagga gtttaaggag gccttcaaca tgattgatca gaaccgcgat
      181 ggcttcattg ataaggagga cctgcacgac atgttggcct ctctggggaa gaaccccaca
      241 gacgagtacc tggagggcat gatgaacgag gcgccagggc ccatcaactt caccatgttc
      301 ctcacaatgt ttggggagaa gctgaacggc acagaccccg aggatgtgat ccgcaatgcc
      361 tttgcctgct ttgatgagga ggcctcaggc ttcatccacg aggaccacct gagggagctg
      421 ctcaccacca tgggcgaccg attcacggat gaggaggtgg acgagatgta ccgcgaggca
      481 cccattgata agaagggcaa cttcaactac gtggagttca ctcgcatcct caaacacggc
      541 gccaaggaca aggacgacct ggatccaccg gtcgccacca tggtgagcaa gggcgaggag
      601 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      661 ttcagcgtga ggggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc
      721 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgagccac
      781 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      841 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcacctac
      901 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      961 ggcgtcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacttcaac
     1021 agccacaaca tctatatcat ggccgtcaag cagaagaacg gcatcaaggt gaacttcaag
     1081 atccgccaca acgtggagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1141 cccatcggcg acggccccgt gctgctgccc gacagccact acctgagcac ccagtccgtg
     1201 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt ccgcaccgcc
     1261 gccgggatca ctctcggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat
     1321 aatcagccat accacatt
//
