LOCUS       sequence-85652-e        2048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 383, Emission: 448.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2048)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBFP2-MAP4-C-10
FEATURES             Location/Qualifiers
     source          1..2048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgaggggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgag ccacggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcac ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcgtc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac
      481 aacatctata tcatggccgt caagcagaag aacggcatca aggtgaactt caagatccgc
      541 cacaacgtgg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacagc cactacctga gcacccagtc cgtgctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttccgcac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctggtgg ctccgcagga
      781 tcccggcaag aagaagcaaa ggctgctgta ggtgtgactg gaaatgacat cactaccccg
      841 ccaaacaagg agccaccacc aagcccagaa aagaaagcaa agcctttggc caccactcaa
      901 cctgcaaaga cttcaacatc gaaagccaaa acacagccca cttctctccc taagcaacca
      961 gctcccacca cctctggtgg gttgaataaa aaacccatga gcctcgcctc aggctcagtg
     1021 ccagctgccc cacacaaacg ccctgctgct gccactgcta ctgccaggcc ttccacccta
     1081 cctgccagag acgtgaagcc aaagccaatt acagaagcta aggttgccga aaagcggacc
     1141 tctccatcca agccttcatc tgccccagcc ctcaaacctg gacctaaaac caccccaacc
     1201 gtttcaaaag ccacatctcc ctcaactctt gtttccactg gaccaagtag tagaagtcca
     1261 gctacaactc tgcctaagag gccaaccagc atcaagactg aggggaaacc tgctgatgtc
     1321 aaaaggatga ctgctaagtc tgcctcagct gacttgagtc gctcaaagac cacctctgcc
     1381 agttctgtga agagaaacac cactcccact ggggcagcac ccccagcagg gatgacttcc
     1441 actcgagtca agcccatgtc tgcacctagc cgctcttctg gggctctttc tgtggacaag
     1501 aagcccactt ccactaagcc tagctcctct gctcccaggg tgagccgcct ggccacaact
     1561 gtttctgccc ctgacctgaa gagtgttcgc tccaaggtcg gctctacaga aaacatcaaa
     1621 caccagcctg gaggaggccg ggccaaggta gagaaaaaaa cagaggcagc taccacagct
     1681 gggaagcctg aacctaatgc agtcactaaa gcagccggct ccattgcgag tgcacagaaa
     1741 ccgcctgctg ggaaagtcca gatagtatcc aaaaaagtga gctacagtca tattcaatcc
     1801 aagtgtgttt ccaaggacaa tattaagcat gtccctggat gtggcaatgt tcagattcag
     1861 aacaagaaag tggacatatc caaggtctcc tccaagtgtg ggtccaaagc taatatcaag
     1921 cacaagcctg gtggaggaga tgtcaagatt gaaagtcaga agttgaactt caaggagaag
     1981 gcccaagcca aagtgggata gggatccacc ggatctagat aactgatcat aatcagccat
     2041 accacatt
//
