LOCUS       sequence-85655-e        1719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 383, Emission: 448.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1719)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBFP2-PCNA-19-SV40NLS-4
FEATURES             Location/Qualifiers
     source          1..1719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..51
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             55..75
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             79..99
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      106..115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(112..114,115..117,118..828)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(157..178)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttgacgtcag atccgctagc caccatggat ccaaaaaaga agagaaaggt agatccaaaa
       61 aagaagagaa aggtagatcc aaaaaagaag agaaaggtac cggtcgccac catggtgagc
      121 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      181 aacggccaca agttcagcgt gaggggcgag ggcgagggcg atgccaccaa cggcaagctg
      241 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      301 accctgagcc acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
      361 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      421 gacggcacct acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      481 atcgagctga agggcgtcga cttcaaggag gacggcaaca tcctggggca caagctggag
      541 tacaacttca acagccacaa catctatatc atggccgtca agcagaagaa cggcatcaag
      601 gtgaacttca agatccgcca caacgtggag gacggcagcg tgcagctcgc cgaccactac
      661 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacagcca ctacctgagc
      721 acccagtccg tgctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      781 ttccgcaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc cggagaaggg
      841 caagggcaag ggcaagggcc gggccgcggc tacgcgtatc gatccatgtt cgaggcgcgc
      901 ctggtccagg gctccatcct caagaaggtg ttggaggcac tcaaggacct catcaacgag
      961 gcctgctggg atattagctc cagcggtgta aacctgcaga gcatggactc gtcccacgtc
     1021 tctttggtgc agctcaccct gcggtctgag ggcttcgaca cctaccgctg cgaccgcaac
     1081 ctggccatgg gcgtgaacct caccagtatg tccaaaatac taaaatgcgc cggcaatgaa
     1141 gatatcatta cactaagggc cgaagataac gcggatacct tggcgctagt atttgaagca
     1201 ccaaaccagg agaaagtttc agactatgaa atgaagttga tggatttaga tgttgaacaa
     1261 cttggaattc cagaacagga gtacagctgt gtagtaaaga tgccttctgg tgaatttgca
     1321 cgtatatgcc gagatctcag ccatattgga gatgctgttg taatttcctg tgcaaaagac
     1381 ggagtgaaat tttctgcaag tggagaactt ggaaatggaa acattaaatt gtcacagaca
     1441 agtaatgtcg ataaagagga ggaagctgtt accatagaga tgaatgaacc agttcaacta
     1501 acttttgcac tgaggtacct gaacttcttt acaaaagcca ctccactctc ttcaacggtg
     1561 acactcagta tgtctgcaga tgtacccctt gttgtagagt ataaaattgc ggatatggga
     1621 cacttaaaat actacttggc tcccaagatc gaggatgaag aaggatctta gggatccacc
     1681 ggatctagat aactgatcat aatcagccat accacattg
//
