LOCUS       sequence-85656-e        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 383, Emission: 448.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBFP2-VASP-5
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(34..36,37..39,40..750)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtgaggggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgag ccacggcgtg
      241 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcac ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcgtc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac
      481 aacatctata tcatggccgt caagcagaag aacggcatca aggtgaactt caagatccgc
      541 cacaacgtgg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacagc cactacctga gcacccagtc cgtgctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttccgcac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctatgag cgagacggtc
      781 atctgttcca gccgggctac tgtgatgctt tatgatgaca gcaacaagcg atggctccct
      841 gctggcactg gtccccaggc cttcagccgc gtccagatat accacaaccc cactgctaac
      901 tccttccgag ttgttggccg caagatgcag ccggatcagc aggtggttat caactgtgcc
      961 atcattcggg gtgtcaagta caatcaggcc actcccatct tccatcagtg gcgagatgcc
     1021 cgccaggtct ggggcctcaa cttcggcagc aaggaggacg ccatacagtt tgcaacaggc
     1081 atggccaacg ccctagaggc cttggaagga ggtgggcctc ccccagcccc agcaccccct
     1141 gcctggtctg cccagaatgg tccctcccca gaggagctgg aacaacagaa aaggcagccg
     1201 gagcatatgg agcgccgggt ctccaatgca ggaggcccac ctgctccccc agctgggggc
     1261 cctcctccac ctccaggacc tccccctcct ccaggtcccc ccccaccccc aggtctgccc
     1321 tcctcagggg tatctggggc aggtcatgga gcaggggcag ccccaccccc tgcaccccca
     1381 ctccctacaa cacagggccc caatagtggg ggttccgggg ccccaggcct ggctgctgcc
     1441 attgctggag ccaaactcag gaaagtgagc aagcaggagg aggcctctgg ggggcccctg
     1501 gcccccaaag ctgagaacag tcgaagcact ggtggggggc ttatggaaga gatgaacgcc
     1561 atgctggccc ggagaagaaa agccacacag gttggggaga agccccccaa agacgagtca
     1621 gccagtgagg agtcagaggc ccgactccct gcccagagtg aacctgtgag aagaccctgg
     1681 gagaagaaca gcacaacctt gccaaggatg aagtcgtcct cttctgtgac tacctccgag
     1741 gcccacccct cgacgccctg ctccagtgat gactccgact tggagagggt gaagcaggag
     1801 cttctggaag aggtgcggaa ggagctacag aaaatgaaag aggaaatcat cgaagtcttt
     1861 gtccaggagc tgaggaagcg gggttctcct tgagcggccg cgactctaga tcataat
//
