LOCUS       sequence-85657-e        4026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 383, Emission: 448.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4026)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBFP2-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..4026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3..12
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9..11,12..14,15..725)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(54..75)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
       61 agctggacgg cgacgtaaac ggccacaagt tcagcgtgag gggcgagggc gagggcgatg
      121 ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      181 ggcccaccct cgtgaccacc ctgagccacg gcgtgcagtg cttcgcccgc taccccgacc
      241 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      301 ccatcttctt caaggacgac ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg
      361 acaccctggt gaaccgcatc gagctgaagg gcgtcgactt caaggaggac ggcaacatcc
      421 tggggcacaa gctggagtac aacttcaaca gccacaacat ctatatcatg gccgtcaagc
      481 agaagaacgg catcaaggtg aacttcaaga tccgccacaa cgtggaggac ggcagcgtgc
      541 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      601 acagccacta cctgagcacc cagtccgtgc tgagcaaaga ccccaacgag aagcgcgatc
      661 acatggtcct gctggagttc cgcaccgccg ccgggatcac tctcggcatg gacgagctgt
      721 acaagtccgg actcagatct cgagctcaag cttcgaattc gggaaactcg agaacaaagc
      781 ttgaaaacgg ccgcatgcca gtgtttcata cgcgcacgat cgagagcatc ctggagccgg
      841 tggcacagca gatctcccac ctggtgataa tgcacgagga gggcgaggtg gacggcaaag
      901 ccattcctga cctcaccgcg cccgtggccg ccgtgcaggc ggccgtcagc aacctcgtcc
      961 gggttggaaa agagactgtt caaaccactg aggatcagat tttgaagaga gatatgccac
     1021 cagcatttat taaggttgag aatgcttgca ccaagcttgt ccaggcagct cagatgcttc
     1081 agtcagaccc ttactcagtg cctgctcgag attatctaat tgatgggtca aggggcatcc
     1141 tctctggaac atcagacctg ctccttacct tcgatgaggc tgaggtccgt aaaattatta
     1201 gagtttgcaa aggaattttg gaatatctta cagtggcaga ggtggtggag actatggaag
     1261 atttggtcac ttacacaaag aatcttgggc caggaatgac taagatggcc aagatgattg
     1321 acgagagaca gcaggagctc actcaccagg agcaccgagt gatgttggtg aactcgatga
     1381 acaccgtgaa agagttgctg ccagttctca tttcagctat gaagattttt gtaacaacta
     1441 aaaactcaaa aaaccaaggc atagaggaag ctttaaaaaa tcgcaatttt actctagaaa
     1501 aaatgagtgc tgaaattaat gagataattc gtgtgttaca actcacctct tgggatgaag
     1561 atgcctgggc cagcaaggac actgaagcca tgaagagagc attggcctcc atagactcca
     1621 aactgaacca ggccaaaggt tggctccgtg accctagtgc ctccccaggg gatgctggtg
     1681 agcaggccat cagacagatc ttagatgaag ctggaaaagt tggtgaactc tgtgcaggca
     1741 aagaacgcag ggagattctg ggaacttgca aaatgctagg gcagatgact gatcaagtgg
     1801 ctgacctccg tgccagagga caaggatcct caccggtggc catgcagaaa gctcagcagg
     1861 tatctcaggg tctggatgtg ctcacagcaa aagtggaaaa tgcagctcgc aagctggaag
     1921 ccatgaccaa ctcaaagcag agcattgcaa agaagatcga tgctgctcag aactggcttg
     1981 cagatccaaa tggtggaccg gaaggagaag agcagattcg aggtgctttg gctgaagctc
     2041 ggaaaatagc agaattatgt gatgatccta aagaaagaga tgacattcta cgttcccttg
     2101 gggaaatatc tgctctgact tctaaattag cagatctacg aagacagggg aaaggagatt
     2161 ctccagaggc tcgagccttg gccaaacagg tggccacggc cctgcagaac ctgcagacca
     2221 aaaccaaccg ggctgtggcc aacagcagac cggccaaagc agctgtacac cttgagggca
     2281 agattgagca agcacagcgg tggattgata atcccacagt ggatgaccgt ggagtcggtc
     2341 aggctgccat ccgggggctt gtggccgaag ggcatcgtct ggctaatgtt atgatggggc
     2401 cttatcggca agatcttctc gccaagtgtg accgagtgga ccagctgaca gcccagctgg
     2461 ctgacctggc tgccagaggg gaaggggaga gtcctcaggc acgagcactt gcatctcagc
     2521 tccaagactc cttaaaggat ctaaaagctc ggatgcagga ggccatgact caggaagtgt
     2581 cagatgtttt cagcgatacc acaactccca tcaagctgtt ggcagtggca gccacggcgc
     2641 ctcctgatgc gcctaacagg gaagaggtat ttgatgagag ggcagctaac tttgaaaacc
     2701 attcaggaaa gcttggtgct acggccgaga aggcggctgc ggttggtact gctaataaat
     2761 caacagtgga aggcattcag gcctcagtga agacggcccg agaactcaca ccccaggtgg
     2821 tctcggctgc tcgtatctta cttaggaacc ctggaaatca agctgcttat gaacattttg
     2881 agaccatgaa gaaccagtgg atcgataatg ttgaaaaaat gacagggctg gtggacgaag
     2941 ccattgatac caaatctctg ttggatgctt cagaagaagc aattaaaaaa gacctggaca
     3001 agtgcaaggt agctatggcc aacattcagc ctcagatgct ggttgctggg gcaaccagta
     3061 ttgctcgtcg ggccaaccgg atcctgctgg tggctaagag ggaggtggag aattccgagg
     3121 atcccaagtt ccgtgaggct gtgaaagctg cctctgatga attgagcaaa accatctccc
     3181 cgatggtgat ggatgcaaaa gctgtggctg gaaacatttc cgaccctgga ctgcaaaaga
     3241 gcttcctgga ctcaggatat cggatcctgg gagctgtggc caaggtcaga gaagccttcc
     3301 aacctcagga gcctgacttc ccgccgcctc caccagacct tgaacaactc cgactaacag
     3361 atgagcttgc tcctcccaaa ccacctctgc ctgaaggtga ggtccctcca cctaggcctc
     3421 caccaccaga ggaaaaggat gaagagttcc ctgagcagaa ggccggggag gtgattaacc
     3481 agccaatgat gatggctgcc agacagctcc atgatgaagc tcgcaaatgg tccagcaagg
     3541 gcaatgacat cattgcagca gccaagcgca tggctctgct gatggctgag atgtctcggc
     3601 tggtaagagg gggcagtggt accaagcggg cactcattca gtgtgccaag gacatcgcca
     3661 aggcctcaga tgaggtgact cggttggcca aggaggttgc caagcagtgc acagataaac
     3721 ggattagaac caacctctta caggtatgtg agcgaatccc aaccataagc acccagctca
     3781 aaatcctgtc cacagtgaag gccaccatgc tgggccggac caacatcagt gatgaggagt
     3841 ctgagcaggc cacagagatg ctggttcaca atgcccagaa cctcatgcag tctgtgaagg
     3901 agactgtgcg ggaagctgaa gctgcttcaa tcaaaattcg aacagatgct ggatttacac
     3961 tgcgctgggt tagaaagact ccctggtacc agtaggcggc cgcgactcta gatcataatc
     4021 agccat
//
