LOCUS       sequence-85668-e        2059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mTagBFP-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(40..102,103..1260)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     CDS             1321..2022
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agcgccacca tggcggcccc gggcgcccgg
       61 cgtccgctgc tcctgttgct gctggcaggc cttgcacaca gcgccccagc actgttcgag
      121 gtgaaagaca acaacggcac agcgtgtata atggccagct tctctgcctc ctttctgacc
      181 acctatgagg ctggacatgt ttctaaggtc tcgaatatga ccctgccagc ctctgcagaa
      241 gtcctgaaga atagcagctc ttgtggtgaa aagaatgctt ctgagcccac cctcgcaatc
      301 acctttggag aaggatattt actgaaactc accttcacaa aaaacacaac acgttacagt
      361 gtccagcaca tgtatttcac atataacctg tcagacacac aattctttcc caatgccagc
      421 tccaaagggc ccgacactgt ggattccaca actgacatca aggcagacat caacaaaaca
      481 taccgatgtg tcagcgacat cagggtctac atgaagaatg tgaccattgt gctctgggac
      541 gctactatcc aggcctacct gccgagtagc aacttcagca aggaagagac acgctgccca
      601 caggatcaac cttccccaac tactgggcca cccagcccct caccaccact tgtgcccaca
      661 aaccccagtg tgtccaagta caatgtgact ggtgacaatg gaacctgcct gctggcctct
      721 atggcactgc aactcaacat cacctacatg aagaaggaca acacgactgt gaccagagca
      781 ttcaacatca acccaagtga caaatatagt gggacttgcg gtgcccagtt ggtgaccctg
      841 aaggtgggga acaagagcag agtcctggag ctgcagtttg ggatgaatgc cacttctagc
      901 ctgtttttcc tgcaaggagt tcagttgaac atgactcttc ctgatgccat agagcccacg
      961 ttcagcacct ccaactattc cctgaaagct cttcaggcca gtgtcggcaa ctcatacaag
     1021 tgcaacagtg aggagcacat ctttgtcagc aaggcgctcg ccctcaatgt cttcagcgtg
     1081 caagtccagg ctttcagggt agaaagtgac aggtttgggt ctgtggaaga gtgtgtacag
     1141 gacggtaaca acatgctgat ccccattgct gtgggcgggg ccctggcagg gctggtcctc
     1201 atcgtcctca tcgcctacct catcggcagg aagaggagtc acgcgggcta tcagaccatc
     1261 tcggaattcg gctccaccgg ctccaccggc tccaccggcg cggatccacc ggtcgccacc
     1321 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     1381 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     1441 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     1501 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     1561 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     1621 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     1681 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     1741 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     1801 gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac atatagatcc
     1861 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     1921 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     1981 tactgcgacc tccctagcaa actggggcac aagcttaatt aaagcggccg cgactctaga
     2041 tcataatcag ccataccac
//
