LOCUS       sequence-85672-e        1444 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1444)
  TITLE     Davidson Blue Fluorescent Proteins
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagBFP-Rab5a-7
FEATURES             Location/Qualifiers
     source          1..1444
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..727
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 gacgtcagat ccgctagcgg tcgccaccat gagcgagctg attaaggaga acatgcacat
       61 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
      121 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
      181 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
      241 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
      301 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
      361 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
      421 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga
      481 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
      541 cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
      601 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
      661 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
      721 gcttaattcc ggactcagat ctcgagccat ggctagtcga ggcgcaacaa gacccaacgg
      781 gccaaatact ggaaataaaa tatgccagtt caaactagta cttctgggag agtctgctgt
      841 tggcaaatca agcctagtgc ttcgttttgt gaagggccaa tttcatgaat ttcaagagag
      901 taccataggg gctgcttttc taacccaaac tgtgtgtctt gatgatacaa cagtaaagtt
      961 tgaaatatgg gatacagctg gtcaagaacg ataccatagc ttagcaccaa tgtactacag
     1021 aggagcacaa gcagccatag ttgtatatga tatcacaaat gaggagtcct ttgccagagc
     1081 caaaaactgg gttaaagaac ttcagaggca agccagtcct aacattgtaa tagctttatc
     1141 aggaaacaag gctgatcttg caaataaaag agctgtcgat ttccaggaag cacagtccta
     1201 tgcagatgac aacagtttat tattcatgga gacatcagct aaaacatcga tgaatgtaaa
     1261 tgaaatattc atggcaatag ctaaaaagtt gccaaagaac gaaccacaga atccaggagc
     1321 aaattctgcc agaggaagag gagtagacct tactgaaccc acgcagccaa ccaggaatca
     1381 gtgttgtagt aactaaggat ccaccggatc tagataactg atcataatca gccataccac
     1441 attt
//
