LOCUS       sequence-85681-e        1793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Annexin-12
FEATURES             Location/Qualifiers
     source          1..1793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..744
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtgt ctaagggcga agagctgatt
       61 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag
      121 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg
      181 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc
      241 agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct
      301 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
      361 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
      421 ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag
      481 acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg
      541 ggcgggagcc atctgatcgc aaacgccaag accacatata gatccaagaa acccgctaag
      601 aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc
      661 aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct
      721 agcaaactgg ggcacaagct taattccgga ctcagatctc gagctcaagc ttcgaattct
      781 atggccatgg caaccaaagg aggtactgtc aaagctgctt caggattcaa tgccatggaa
      841 gatgcccaga ccctgaggaa ggccatgaaa gggctcggca ccgatgaaga cgccattatt
      901 agcgtccttg cctaccgcaa caccgcccag cgccaggaga tcaggacagc ctacaagagc
      961 accatcggca gggacttgat agacgacctg aagtcagaac tgagtggcaa cttcgagcag
     1021 gtgattgtgg ggatgatgac gcccacggtg ctgtatgacg tgcaagagct gcgaagggcc
     1081 atgaagggag ccggcactga tgagggctgc ctaattgaga tcctggcctc ccggacccct
     1141 gaggagatcc ggcgcataag ccaaacctac cagcagcaat atggacggag ccttgaagat
     1201 gacattcgct ctgacacatc gttcatgttc cagcgagtgc tggtgtctct gtcagctggt
     1261 gggagggatg aaggaaatta tctggacgat gctctcgtga gacaggatgc ccaggacctg
     1321 tatgaggctg gagagaagaa atgggggaca gatgaggtga aatttctaac tgttctctgt
     1381 tcccggaacc gaaatcacct gttgcatgtg tttgatgaat acaaaaggat atcacagaag
     1441 gatattgaac agagtattaa atctgaaaca tctggtagct ttgaagatgc tctgctggct
     1501 atagtaaagt gcatgaggaa caaatctgca tattttgctg aaaagctcta taaatcgatg
     1561 aagggcttgg gcaccgatga taacaccctc atcagagtga tggtttctcg agcagaaatt
     1621 gacatgttgg atatccgggc acacttcaag agactctatg gaaagtctct gtactcgttc
     1681 atcaagggtg acacatctgg agactacagg aaagtactgc ttgttctctg tggaggagat
     1741 gattaaggat ccaccggatc tagataactg atcataatca gccataccac att
//
