LOCUS       sequence-85684-e        1381 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-Caveolin-C-10
FEATURES             Location/Qualifiers
     source          1..1381
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..737
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 cgtcagatcc gctagcggtg gccaccatgg tgtctaaggg cgaagagctg attaaggaga
       61 acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat
      121 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg
      181 gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga
      241 ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct
      301 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
      361 ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat
      421 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt
      481 accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga
      541 gccatctgat cgcaaacgcc aagaccacat atagatccaa gaaacccgct aagaacctca
      601 agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg
      661 agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac
      721 tggggcacaa gcttaattcc ggactcagat ctggcagcag tggaggttct gggggcaaat
      781 acgtagactc ggagggacat ctctacaccg ttcccatccg ggaacagggc aacatctaca
      841 agcccaacaa caaggccatg gcagacgagc tgagcgagaa gcaagtgtac gacgcgcaca
      901 ccaaggagat cgacctggtc aaccgcgacc ctaaacacct caacgatgac gtggtcaaga
      961 ttgactttga agatgtgatt gcagaaccag aagggacaca cagttttgac ggcatttgga
     1021 aggccagctt caccaccttc actgtgacga aatactggtt ttaccgcttg ctgtctgccc
     1081 tctttggcat cccgatggca ctcatctggg gcatttactt cgccattctc tctttcctgc
     1141 acatctgggc agttgtacca tgcattaaga gcttcctgat tgagattcag tgcatcagcc
     1201 gtgtctattc catctacgtc cacaccgtct gtgacccact ctttgaagct gttgggaaaa
     1261 tattcagcaa tgtccgcatc aacttgcaga aagaaatata agaattctgc agtcgacggt
     1321 accgcgggcc cgggatccac cggatctaga taactgatca taatcagcca taccacattt
     1381 t
//
