LOCUS       sequence-85687-e        1589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tetraspanin/Membrane, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-CD151-7
FEATURES             Location/Qualifiers
     source          1..1589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..738
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 acgtcagatc cgctagcggt ggccaccatg gtgtctaagg gcgaagagct gattaaggag
       61 aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca
      121 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag
      181 ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag
      241 accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc
      301 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
      361 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca
      421 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg
      481 taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg
      541 agccatctga tcgcaaacgc caagaccaca tatagatcca agaaacccgc taagaacctc
      601 aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac
      661 gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa
      721 ctggggcaca agcttaattc cggactcaga tctggcggta tgggtgagtt caacgagaag
      781 aagacaacat gtggcaccgt ttgcctcaag tacctgctgt ttacctacaa ttgctgcttc
      841 tggctggctg gcctggctgt catggcagtg ggcatctgga cgctggccct caagagtgac
      901 tacatcagcc tgctggcctc aggcacctac ctggccacag cctacatcct ggtggtggcg
      961 ggcactgtcg tcatggtgac tggggtcttg ggctgctgcg ccaccttcaa ggagcgtcgg
     1021 aacctgctgc gcctgtactt catcctgctc ctcatcatct ttctgctgga gatcatcgct
     1081 ggtatcctcg cctacgccta ctaccagcag ctgaacacgg agctcaagga gaacctgaag
     1141 gacaccatga ccaagcgcta ccaccagccg ggccatgagg ctgtgaccag cgctgtggac
     1201 cagctgcagc aggagttcca ctgctgtggc agcaacaact cacaggactg gcgagacagt
     1261 gagtggatcc gctcacagga ggccggtggc cgtgtggtcc cagacagctg ctgcaagacg
     1321 gtggtggctc tttgtggaca gcgagaccat gcctccaaca tctacaaggt ggagggcggc
     1381 tgcatcacca agttggagac cttcatccag gagcacctga gggtcattgg ggctgtgggg
     1441 atcggcattg cctgtgtgca ggtctttggc atgatcttca cgtgctgcct gtacaggagt
     1501 ctcaagctgg agcactactg agaattctgc agtcgacggt accgcgggcc cgggatccac
     1561 cggatctaga taactgatca taatcagcc
//
